Loading…

Pseudomonas fluorescens NZI7 repels grazing by C. elegans, a natural predator

The bacteriovorous nematode Caenorhabditis elegans has been used to investigate many aspects of animal biology, including interactions with pathogenic bacteria. However, studies examining C. elegans interactions with bacteria isolated from environments in which it is found naturally are relatively s...

Full description

Saved in:
Bibliographic Details
Published in:The ISME Journal 2013-06, Vol.7 (6), p.1126-1138
Main Authors: Burlinson, Peter, Studholme, David, Cambray-Young, Joanna, Heavens, Darren, Rathjen, John, Hodgkin, Jonathan, Preston, Gail M
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c549t-63502f82a13ae9f52cb04726150832c01df55a7095eb768e736e44d6a4566e693
cites cdi_FETCH-LOGICAL-c549t-63502f82a13ae9f52cb04726150832c01df55a7095eb768e736e44d6a4566e693
container_end_page 1138
container_issue 6
container_start_page 1126
container_title The ISME Journal
container_volume 7
creator Burlinson, Peter
Studholme, David
Cambray-Young, Joanna
Heavens, Darren
Rathjen, John
Hodgkin, Jonathan
Preston, Gail M
description The bacteriovorous nematode Caenorhabditis elegans has been used to investigate many aspects of animal biology, including interactions with pathogenic bacteria. However, studies examining C. elegans interactions with bacteria isolated from environments in which it is found naturally are relatively scarce. C. elegans is frequently associated with cultivation of the edible mushroom Agaricus bisporus, and has been reported to increase the severity of bacterial blotch of mushrooms, a disease caused by bacteria from the Pseudomonas fluorescens complex. We observed that pseudomonads isolated from mushroom farms showed differential resistance to nematode predation. Under nutrient poor conditions, in which most pseudomonads were consumed, the mushroom pathogenic isolate P. fluorescens NZI7 was able to repel C. elegans without causing nematode death. A draft genome sequence of NZI7 showed it to be related to the biocontrol strain P. protegens Pf-5. To identify the genetic basis of nematode repellence in NZI7, we developed a grid-based screen for mutants that lacked the ability to repel C. elegans . The mutants isolated in this screen included strains with insertions in the global regulator GacS and in a previously undescribed GacS-regulated gene cluster, ‘EDB’ (‘edible’). Our results suggest that the product of the EDB cluster is a poorly diffusible or cell-associated factor that acts together with other features of NZI7 to provide a novel mechanism to deter nematode grazing. As nematodes interact with NZI7 colonies before being repelled, the EDB factor may enable NZI7 to come into contact with and be disseminated by C. elegans without being subject to intensive predation.
doi_str_mv 10.1038/ismej.2013.9
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3660686</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1367488975</sourcerecordid><originalsourceid>FETCH-LOGICAL-c549t-63502f82a13ae9f52cb04726150832c01df55a7095eb768e736e44d6a4566e693</originalsourceid><addsrcrecordid>eNqNkUtv1DAUhS1ExZQpO9bIEhsWk9SP2E42SNWIR6W2sGg33VhOchMySuxgJ0jl1-N5MBoQC1bX0v107jk-CL2mJKWE55ddGGCTMkJ5WjxD51QJmiiuyPPjW7IFehnChhChpFQv0ILxjElC2Tm6_Rpgrt3grAm46WfnIVRgA757vFbYwwh9wK03Pzvb4vIJr1MMPbTGhhU22Jpp9qbHo4faTM5foLPG9AFeHeYSPXz8cL_-nNx8-XS9vrpJKpEVUyK5IKzJmaHcQNEIVpUkU0xSQXLOKkLrRgijSCGgVDKHGAGyrJYmE1KCLPgSvd_rjnM5QB0NT9GGHn03GP-knen0nxvbfdOt-6G5lETmMgq8Owh4932GMOmhi7n73lhwc9CUS5XleaHEf6CCF7nICI_o27_QjZu9jT-xozgjQtBIrfZU5V0IHpqjb0r0tlK9q1RvK9XbrG9Osx7h3x1GINkDIa5sC_7k6r8EfwHGEqrc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1353320551</pqid></control><display><type>article</type><title>Pseudomonas fluorescens NZI7 repels grazing by C. elegans, a natural predator</title><source>OUP_牛津大学出版社OA刊</source><source>PubMed Central</source><creator>Burlinson, Peter ; Studholme, David ; Cambray-Young, Joanna ; Heavens, Darren ; Rathjen, John ; Hodgkin, Jonathan ; Preston, Gail M</creator><creatorcontrib>Burlinson, Peter ; Studholme, David ; Cambray-Young, Joanna ; Heavens, Darren ; Rathjen, John ; Hodgkin, Jonathan ; Preston, Gail M</creatorcontrib><description>The bacteriovorous nematode Caenorhabditis elegans has been used to investigate many aspects of animal biology, including interactions with pathogenic bacteria. However, studies examining C. elegans interactions with bacteria isolated from environments in which it is found naturally are relatively scarce. C. elegans is frequently associated with cultivation of the edible mushroom Agaricus bisporus, and has been reported to increase the severity of bacterial blotch of mushrooms, a disease caused by bacteria from the Pseudomonas fluorescens complex. We observed that pseudomonads isolated from mushroom farms showed differential resistance to nematode predation. Under nutrient poor conditions, in which most pseudomonads were consumed, the mushroom pathogenic isolate P. fluorescens NZI7 was able to repel C. elegans without causing nematode death. A draft genome sequence of NZI7 showed it to be related to the biocontrol strain P. protegens Pf-5. To identify the genetic basis of nematode repellence in NZI7, we developed a grid-based screen for mutants that lacked the ability to repel C. elegans . The mutants isolated in this screen included strains with insertions in the global regulator GacS and in a previously undescribed GacS-regulated gene cluster, ‘EDB’ (‘edible’). Our results suggest that the product of the EDB cluster is a poorly diffusible or cell-associated factor that acts together with other features of NZI7 to provide a novel mechanism to deter nematode grazing. As nematodes interact with NZI7 colonies before being repelled, the EDB factor may enable NZI7 to come into contact with and be disseminated by C. elegans without being subject to intensive predation.</description><identifier>ISSN: 1751-7362</identifier><identifier>EISSN: 1751-7370</identifier><identifier>DOI: 10.1038/ismej.2013.9</identifier><identifier>PMID: 23426012</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/158/855 ; Agaricales ; Agaricus bisporus ; Animals ; Bacteria ; Biological control ; Biomedical and Life Sciences ; Caenorhabditis elegans ; Caenorhabditis elegans - physiology ; Cultivation ; Ecology ; Evolutionary Biology ; Food Chain ; Grazing ; Life Sciences ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Mutants ; Mutation ; Nematoda ; Nematodes ; Nutrients ; Original ; original-article ; Pest Control, Biological ; Pseudomonas fluorescens ; Pseudomonas fluorescens - classification ; Pseudomonas fluorescens - genetics ; Pseudomonas fluorescens - metabolism</subject><ispartof>The ISME Journal, 2013-06, Vol.7 (6), p.1126-1138</ispartof><rights>International Society for Microbial Ecology 2013</rights><rights>Copyright Nature Publishing Group Jun 2013</rights><rights>Copyright © 2013 International Society for Microbial Ecology 2013 International Society for Microbial Ecology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-63502f82a13ae9f52cb04726150832c01df55a7095eb768e736e44d6a4566e693</citedby><cites>FETCH-LOGICAL-c549t-63502f82a13ae9f52cb04726150832c01df55a7095eb768e736e44d6a4566e693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660686/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660686/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23426012$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burlinson, Peter</creatorcontrib><creatorcontrib>Studholme, David</creatorcontrib><creatorcontrib>Cambray-Young, Joanna</creatorcontrib><creatorcontrib>Heavens, Darren</creatorcontrib><creatorcontrib>Rathjen, John</creatorcontrib><creatorcontrib>Hodgkin, Jonathan</creatorcontrib><creatorcontrib>Preston, Gail M</creatorcontrib><title>Pseudomonas fluorescens NZI7 repels grazing by C. elegans, a natural predator</title><title>The ISME Journal</title><addtitle>ISME J</addtitle><addtitle>ISME J</addtitle><description>The bacteriovorous nematode Caenorhabditis elegans has been used to investigate many aspects of animal biology, including interactions with pathogenic bacteria. However, studies examining C. elegans interactions with bacteria isolated from environments in which it is found naturally are relatively scarce. C. elegans is frequently associated with cultivation of the edible mushroom Agaricus bisporus, and has been reported to increase the severity of bacterial blotch of mushrooms, a disease caused by bacteria from the Pseudomonas fluorescens complex. We observed that pseudomonads isolated from mushroom farms showed differential resistance to nematode predation. Under nutrient poor conditions, in which most pseudomonads were consumed, the mushroom pathogenic isolate P. fluorescens NZI7 was able to repel C. elegans without causing nematode death. A draft genome sequence of NZI7 showed it to be related to the biocontrol strain P. protegens Pf-5. To identify the genetic basis of nematode repellence in NZI7, we developed a grid-based screen for mutants that lacked the ability to repel C. elegans . The mutants isolated in this screen included strains with insertions in the global regulator GacS and in a previously undescribed GacS-regulated gene cluster, ‘EDB’ (‘edible’). Our results suggest that the product of the EDB cluster is a poorly diffusible or cell-associated factor that acts together with other features of NZI7 to provide a novel mechanism to deter nematode grazing. As nematodes interact with NZI7 colonies before being repelled, the EDB factor may enable NZI7 to come into contact with and be disseminated by C. elegans without being subject to intensive predation.</description><subject>631/158/855</subject><subject>Agaricales</subject><subject>Agaricus bisporus</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Biological control</subject><subject>Biomedical and Life Sciences</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - physiology</subject><subject>Cultivation</subject><subject>Ecology</subject><subject>Evolutionary Biology</subject><subject>Food Chain</subject><subject>Grazing</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Nematoda</subject><subject>Nematodes</subject><subject>Nutrients</subject><subject>Original</subject><subject>original-article</subject><subject>Pest Control, Biological</subject><subject>Pseudomonas fluorescens</subject><subject>Pseudomonas fluorescens - classification</subject><subject>Pseudomonas fluorescens - genetics</subject><subject>Pseudomonas fluorescens - metabolism</subject><issn>1751-7362</issn><issn>1751-7370</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqNkUtv1DAUhS1ExZQpO9bIEhsWk9SP2E42SNWIR6W2sGg33VhOchMySuxgJ0jl1-N5MBoQC1bX0v107jk-CL2mJKWE55ddGGCTMkJ5WjxD51QJmiiuyPPjW7IFehnChhChpFQv0ILxjElC2Tm6_Rpgrt3grAm46WfnIVRgA757vFbYwwh9wK03Pzvb4vIJr1MMPbTGhhU22Jpp9qbHo4faTM5foLPG9AFeHeYSPXz8cL_-nNx8-XS9vrpJKpEVUyK5IKzJmaHcQNEIVpUkU0xSQXLOKkLrRgijSCGgVDKHGAGyrJYmE1KCLPgSvd_rjnM5QB0NT9GGHn03GP-knen0nxvbfdOt-6G5lETmMgq8Owh4932GMOmhi7n73lhwc9CUS5XleaHEf6CCF7nICI_o27_QjZu9jT-xozgjQtBIrfZU5V0IHpqjb0r0tlK9q1RvK9XbrG9Osx7h3x1GINkDIa5sC_7k6r8EfwHGEqrc</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Burlinson, Peter</creator><creator>Studholme, David</creator><creator>Cambray-Young, Joanna</creator><creator>Heavens, Darren</creator><creator>Rathjen, John</creator><creator>Hodgkin, Jonathan</creator><creator>Preston, Gail M</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130601</creationdate><title>Pseudomonas fluorescens NZI7 repels grazing by C. elegans, a natural predator</title><author>Burlinson, Peter ; Studholme, David ; Cambray-Young, Joanna ; Heavens, Darren ; Rathjen, John ; Hodgkin, Jonathan ; Preston, Gail M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-63502f82a13ae9f52cb04726150832c01df55a7095eb768e736e44d6a4566e693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>631/158/855</topic><topic>Agaricales</topic><topic>Agaricus bisporus</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Biological control</topic><topic>Biomedical and Life Sciences</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - physiology</topic><topic>Cultivation</topic><topic>Ecology</topic><topic>Evolutionary Biology</topic><topic>Food Chain</topic><topic>Grazing</topic><topic>Life Sciences</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Nematoda</topic><topic>Nematodes</topic><topic>Nutrients</topic><topic>Original</topic><topic>original-article</topic><topic>Pest Control, Biological</topic><topic>Pseudomonas fluorescens</topic><topic>Pseudomonas fluorescens - classification</topic><topic>Pseudomonas fluorescens - genetics</topic><topic>Pseudomonas fluorescens - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burlinson, Peter</creatorcontrib><creatorcontrib>Studholme, David</creatorcontrib><creatorcontrib>Cambray-Young, Joanna</creatorcontrib><creatorcontrib>Heavens, Darren</creatorcontrib><creatorcontrib>Rathjen, John</creatorcontrib><creatorcontrib>Hodgkin, Jonathan</creatorcontrib><creatorcontrib>Preston, Gail M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The ISME Journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burlinson, Peter</au><au>Studholme, David</au><au>Cambray-Young, Joanna</au><au>Heavens, Darren</au><au>Rathjen, John</au><au>Hodgkin, Jonathan</au><au>Preston, Gail M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pseudomonas fluorescens NZI7 repels grazing by C. elegans, a natural predator</atitle><jtitle>The ISME Journal</jtitle><stitle>ISME J</stitle><addtitle>ISME J</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>7</volume><issue>6</issue><spage>1126</spage><epage>1138</epage><pages>1126-1138</pages><issn>1751-7362</issn><eissn>1751-7370</eissn><abstract>The bacteriovorous nematode Caenorhabditis elegans has been used to investigate many aspects of animal biology, including interactions with pathogenic bacteria. However, studies examining C. elegans interactions with bacteria isolated from environments in which it is found naturally are relatively scarce. C. elegans is frequently associated with cultivation of the edible mushroom Agaricus bisporus, and has been reported to increase the severity of bacterial blotch of mushrooms, a disease caused by bacteria from the Pseudomonas fluorescens complex. We observed that pseudomonads isolated from mushroom farms showed differential resistance to nematode predation. Under nutrient poor conditions, in which most pseudomonads were consumed, the mushroom pathogenic isolate P. fluorescens NZI7 was able to repel C. elegans without causing nematode death. A draft genome sequence of NZI7 showed it to be related to the biocontrol strain P. protegens Pf-5. To identify the genetic basis of nematode repellence in NZI7, we developed a grid-based screen for mutants that lacked the ability to repel C. elegans . The mutants isolated in this screen included strains with insertions in the global regulator GacS and in a previously undescribed GacS-regulated gene cluster, ‘EDB’ (‘edible’). Our results suggest that the product of the EDB cluster is a poorly diffusible or cell-associated factor that acts together with other features of NZI7 to provide a novel mechanism to deter nematode grazing. As nematodes interact with NZI7 colonies before being repelled, the EDB factor may enable NZI7 to come into contact with and be disseminated by C. elegans without being subject to intensive predation.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>23426012</pmid><doi>10.1038/ismej.2013.9</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1751-7362
ispartof The ISME Journal, 2013-06, Vol.7 (6), p.1126-1138
issn 1751-7362
1751-7370
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3660686
source OUP_牛津大学出版社OA刊; PubMed Central
subjects 631/158/855
Agaricales
Agaricus bisporus
Animals
Bacteria
Biological control
Biomedical and Life Sciences
Caenorhabditis elegans
Caenorhabditis elegans - physiology
Cultivation
Ecology
Evolutionary Biology
Food Chain
Grazing
Life Sciences
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Mutants
Mutation
Nematoda
Nematodes
Nutrients
Original
original-article
Pest Control, Biological
Pseudomonas fluorescens
Pseudomonas fluorescens - classification
Pseudomonas fluorescens - genetics
Pseudomonas fluorescens - metabolism
title Pseudomonas fluorescens NZI7 repels grazing by C. elegans, a natural predator
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T00%3A13%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pseudomonas%20fluorescens%20NZI7%20repels%20grazing%20by%20C.%20elegans,%20a%20natural%20predator&rft.jtitle=The%20ISME%20Journal&rft.au=Burlinson,%20Peter&rft.date=2013-06-01&rft.volume=7&rft.issue=6&rft.spage=1126&rft.epage=1138&rft.pages=1126-1138&rft.issn=1751-7362&rft.eissn=1751-7370&rft_id=info:doi/10.1038/ismej.2013.9&rft_dat=%3Cproquest_pubme%3E1367488975%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c549t-63502f82a13ae9f52cb04726150832c01df55a7095eb768e736e44d6a4566e693%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1353320551&rft_id=info:pmid/23426012&rfr_iscdi=true