Mass spectrometry identification of granins and other proteins secreted by neuroblastoma cells

We used mass spectrometry-based protein identification to determine the presence of granins and other proteins in the mouse neuroblastoma secretome. We detected polypeptides derived from four members of the granin family: chromogranin A, chromogranin B, secretogranin III, and VGF. Many of them are d...

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Bibliographic Details
Published in:Tumor biology 2013-06, Vol.34 (3), p.1773-1781
Main Authors: Rozek, Wojciech, Kwasnik, Malgorzata, Debski, Janusz, Zmudzinski, Jan F.
Format: Article
Language:English
Subjects:
Algorithms
Amino Acid Sequence
Animals
Apoptosis
Biomarkers, Tumor - metabolism
Biomedical and Life Sciences
Biomedicine
Blotting, Western
Cancer
Cancer Research
Cell Adhesion
Cell Cycle
Cell Movement
Cell Proliferation
Cellular biology
Chromatography, Liquid
Chromogranins - metabolism
Mass spectrometry
Mice
Molecular Sequence Data
Nervous system
Neuroblastoma - metabolism
Peptide Fragments - analysis
Proteins
Proteins - metabolism
Proteome - metabolism
Research Article
Rodents
Sequence Homology, Amino Acid
Tandem Mass Spectrometry
Tumor Cells, Cultured
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Summary:We used mass spectrometry-based protein identification to determine the presence of granins and other proteins in the mouse neuroblastoma secretome. We detected polypeptides derived from four members of the granin family: chromogranin A, chromogranin B, secretogranin III, and VGF. Many of them are derived from previously described biologically active regions; however, for VGF and CgB, we detected peptides not related to known bioactivities. Along with granins, we identified 115 other proteins secreted by mouse neuroblastoma cells, belonging to different functional categories. Fifty-six out of 119 detected proteins possess the signal fragments required for translocation into endoplasmic reticulum. Sequences of remaining 63 proteins were analyzed using SecretomeP algorithm to determine probability of nonclassical secretion. Identified proteins are involved in the regulation of cell cycle, proliferation, apoptosis, angiogenesis, proteolysis, and cell adhesion.
ISSN:1010-4283
1423-0380
DOI:10.1007/s13277-013-0716-0