Inclusion Complex of Zerumbone with Hydroxypropyl-β-Cyclodextrin Induces Apoptosis in Liver Hepatocellular HepG2 Cells via Caspase 8/BID Cleavage Switch and Modulating Bcl2/Bax Ratio

Zerumbone (ZER) isolated from Zingiber zerumbet was previously encapsulated with hydroxypropyl-β-cyclodextrin (HPβCD) to enhance ZER’s solubility in water, thus making it highly tolerable in the human body. The anticancer effects of this new ZER-HPβCD inclusion complex via apoptosis cell death were...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2013-01, Vol.2013 (2013), p.1-16
Main Authors: Abdul, Ahmad Bustamam, Muhammad Nadzri, Nabilah, Sukari, Mohd Aspollah, Abdel Wahab, Siddig Ibrahim, Eid, Eltayeb E. M., Mohan, Syam, Kamalidehghan, Behnam, Anasamy, Theebaa, Ng, Kuan Beng, Syam, Suvitha, Arbab, Ismail Adam, Rahman, Heshu Sulaiman, Ali, Hapipah Mohd
Format: Article
Language:English
Subjects:
Apoptosis
Bcl-2 protein
Breast cancer
Cancer therapies
Caspase
Caspase-3
Caspase-8
Caspase-9
Cell death
Cytochrome
Cytochrome c
Cytotoxicity
Fourier transforms
Heat shock proteins
Hepatocellular carcinoma
Hepatocytes
Liver
Liver cancer
Medical research
Membrane potential
Microscopy
Mitochondria
Morphology
Zerumbone
β-Cyclodextrin
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Summary:Zerumbone (ZER) isolated from Zingiber zerumbet was previously encapsulated with hydroxypropyl-β-cyclodextrin (HPβCD) to enhance ZER’s solubility in water, thus making it highly tolerable in the human body. The anticancer effects of this new ZER-HPβCD inclusion complex via apoptosis cell death were assessed in this study for the first time in liver hepatocellular cells, HepG2. Apoptosis was ascertained by morphological study, nuclear stain, and sub-G1 cell population accumulation with G2/M arrest. Further investigations showed the release of cytochrome c and loss of mitochondrial membrane potential, proving mitochondrial dysfunction upon the ZER-HPβCD treatment as well as modulating proapoptotic and anti-apototic Bcl-2 family members. A significant increase in caspase 3/7, caspase 9, and caspase 8 was detected with the depletion of BID cleaved by caspase 8. Collectively, these results prove that a highly soluble inclusion complex of ZER-HPβCD could be a promising anticancer agent for the treatment of hepatocellular carcinoma in humans.
ISSN:1741-427X
1741-4288
DOI:10.1155/2013/810632