structure of plant cell walls. III. A model of the walls of suspension-cultured sycamore cells based on the interconnections of the macromolecular components

Degradative enzymes have been used to obtain defined fragments of the isolated cell walls of suspension-cultured sycamore cells. These fragments have been purified and structurally characterized. Fragments released from endopolygalacturonase-pretreated cell walls by a purified endoglucanase and the...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1973, Vol.51 (1), p.188-197
Main Authors: Keegstra, K, Talmadge, K.W, Bauer, W.D, Albersheim, P
Format: Article
Language:English
Subjects:
Acer pseudoplatanus
Carbohydrates
Cell walls
Chemical suspensions
Plant cells
Plants
Polymers
Polysaccharides
Proteins
Sugars
Uronic acids
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Summary:Degradative enzymes have been used to obtain defined fragments of the isolated cell walls of suspension-cultured sycamore cells. These fragments have been purified and structurally characterized. Fragments released from endopolygalacturonase-pretreated cell walls by a purified endoglucanase and the fragments extracted from these walls by urea and alkali provide evidence for a covalent connection between the xyloglucan and pectic polysaccharides. Fragments released by a protease from endopolygalacturonase-endoglucanase-pretreated cell walls provide evidence for a covalent connection between the pectic polysaccharides and the structural protein of the cell wall. Based on these interconnections and the strong binding which occurs between the xyloglucan and cellulose, a tentative structure of the cell wall is proposed.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.51.1.188