Genomic and Proteomic Dissection of the Ubiquitous Plant Pathogen, Armillaria mellea: Toward a New Infection Model System

Armillaria mellea is a major plant pathogen. Yet, no large-scale “-omics” data are available to enable new studies, and limited experimental models are available to investigate basidiomycete pathogenicity. Here we reveal that the A. mellea genome comprises 58.35 Mb, contains 14473 gene models, of av...

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Bibliographic Details
Published in:Journal of proteome research 2013-06, Vol.12 (6), p.2552-2570
Main Authors: Collins, Cassandra, Keane, Thomas M, Turner, Daniel J, O’Keeffe, Grainne, Fitzpatrick, David A, Doyle, Sean
Format: Article
Language:English
Subjects:
Antibiosis
Armillaria - classification
Armillaria - genetics
Armillaria - metabolism
Armillaria - pathogenicity
Candida albicans - growth & development
Chromatography, Liquid
Fungal Proteins - genetics
Fungal Proteins - metabolism
Genome Size
Genome, Fungal
Laccase - isolation & purification
Mycelium - classification
Mycelium - genetics
Mycelium - metabolism
Mycelium - pathogenicity
Peroxidases - isolation & purification
Phylogeny
Plants - microbiology
Proteomics
Species Specificity
Tandem Mass Spectrometry
Virulence
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Summary:Armillaria mellea is a major plant pathogen. Yet, no large-scale “-omics” data are available to enable new studies, and limited experimental models are available to investigate basidiomycete pathogenicity. Here we reveal that the A. mellea genome comprises 58.35 Mb, contains 14473 gene models, of average length 1575 bp (4.72 introns/gene). Tandem mass spectrometry identified 921 mycelial (n = 629 unique) and secreted (n = 183 unique) proteins. Almost 100 mycelial proteins were either species-specific or previously unidentified at the protein level. A number of proteins (n = 111) was detected in both mycelia and culture supernatant extracts. Signal sequence occurrence was 4-fold greater for secreted (50.2%) compared to mycelial (12%) proteins. Analyses revealed a rich reservoir of carbohydrate degrading enzymes, laccases, and lignin peroxidases in the A. mellea proteome, reminiscent of both basidiomycete and ascomycete glycodegradative arsenals. We discovered that A. mellea exhibits a specific killing effect against Candida albicans during coculture. Proteomic investigation of this interaction revealed the unique expression of defensive and potentially offensive A. mellea proteins (n = 30). Overall, our data reveal new insights into the origin of basidiomycete virulence and we present a new model system for further studies aimed at deciphering fungal pathogenic mechanisms.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr301131t