Interaction of colloidal gold nanoparticles with human blood: effects on particle size and analysis of plasma protein binding profiles

Abstract Nanoparticle size and plasma binding profile contribute to a particle's longevity in the bloodstream, which can have important consequences for therapeutic efficacy. In this study an approximate doubling in nanoparticle hydrodynamic size was observed upon in vitro incubation of 30- and...

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Bibliographic Details
Published in:Nanomedicine 2009-06, Vol.5 (2), p.106-117
Main Authors: Dobrovolskaia, Marina A., PhD, Patri, Anil K., PhD, Zheng, Jiwen, PhD, Clogston, Jeffrey D., PhD, Ayub, Nader, Aggarwal, Parag, PhD, Neun, Barry W., BS, Hall, Jennifer B., PhD, McNeil, Scott E., PhD
Format: Article
Language:English
Subjects:
Binding Sites
Blood Proteins - chemistry
Blood Proteins - metabolism
Complement
Gold - chemistry
Gold colloids
Humans
Hydrodynamic size
Internal Medicine
Metal Nanoparticles - chemistry
Microscopy, Electron
Microscopy, Scanning Probe
Nanoparticles
Particle Size
Plasma protein binding
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Summary:Abstract Nanoparticle size and plasma binding profile contribute to a particle's longevity in the bloodstream, which can have important consequences for therapeutic efficacy. In this study an approximate doubling in nanoparticle hydrodynamic size was observed upon in vitro incubation of 30- and 50-nm colloidal gold in human plasma. Plasma proteins that bind the surface of citrate-stabilized gold colloids have been identified. Effects of protein binding on the nanoparticle hydrodynamic size, elements of coagulation, and the complement system have been investigated. The difference in size measurements obtained from dynamic light scattering, electron microscopy, and scanning probe microscopy are also discussed.
ISSN:1549-9634
1549-9642
1549-9642
DOI:10.1016/j.nano.2008.08.001