ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

Highlights • ZFNs, TALENs, and CRISPR/Cas-based RNA-guided DNA endonucleases are programmable site-specific nucleases. • Site-specific nucleases induce DNA DSBs that stimulate NHEJ and HDR at targeted genomic loci. • We discuss the therapeutic potential of site-specific nuclease technologies and dis...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2013-07, Vol.31 (7), p.397-405
Main Authors: Gaj, Thomas, Gersbach, Charles A, Barbas, Carlos F
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological research
Biological Therapy - methods
Biological Therapy - trends
Biotechnology
chimerism
Clustered Regularly Interspaced Short Palindromic Repeats
clustered regulatory interspaced short palindromic repeat
Deoxyribonucleases - genetics
Deoxyribonucleases - metabolism
Deoxyribonucleic acid
DNA
DNA damage
DNA repair
Fundamental and applied biological sciences. Psychology
Genes
Genetic engineering
Genetic Engineering - methods
Genetic Engineering - trends
genetic techniques and protocols
genome engineering
Internal Medicine
Medical research
Methods
Molecular Biology - methods
Molecular Biology - trends
nucleases
Reagents
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
transcription activator-like effector nuclease
Zinc Fingers
zinc-finger nuclease
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Summary:Highlights • ZFNs, TALENs, and CRISPR/Cas-based RNA-guided DNA endonucleases are programmable site-specific nucleases. • Site-specific nucleases induce DNA DSBs that stimulate NHEJ and HDR at targeted genomic loci. • We discuss the therapeutic potential of site-specific nuclease technologies and discuss future prospects for the field.
ISSN:0167-7799
1879-3096
DOI:10.1016/j.tibtech.2013.04.004