Lateral root development in the maize (Zea mays) lateral rootless1 mutant

• Published in: Annals of botany 2013-07, Vol.112 (2), p.417-428
• Main Authors: Husakova, Eva, Hochholdinger, Frank, Soukup, Ales
• Format: Article
• Language: English
• Subjects: apical meristems; Cell Wall - metabolism; corn; cortex; Environment; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; genes; Hydroponics; lignification; lignin; Lignin - metabolism; Meristem - cytology; Meristem - genetics; Meristem - growth & development; mutants; Mutation; permeability; Plant Epidermis - anatomy & histology; Plant Epidermis - genetics; Plant Epidermis - growth & development; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - cytology; Plant Roots - genetics; Plant Roots - growth & development; Plant Shoots - cytology; Plant Shoots - genetics; Plant Shoots - growth & development; Polyphenols - metabolism; root systems; roots; Seedlings - cytology; Seedlings - genetics; Seedlings - growth & development; Zea mays; Zea mays - cytology; Zea mays - genetics; Zea mays - growth & development
• ISSN: 0305-7364; 1095-8290
• DOI: 10.1093/aob/mct043

Background and AimsThe maize lrt1 (lateral rootless1) mutant is impaired in its development of lateral roots during early post-embryonic development. The aim of this study was to characterize, in detail, the influences that the mutation exerts on lateral root initiation and the subsequent developments, as well as to describe the behaviour of the entire plant under variable environmental conditions.MethodsMutant lrt1 plants were cultivated under different conditions of hydroponics, and in between sheets of moist paper. Cleared whole mounts and anatomical sections were used in combination with both selected staining procedures and histochemical tests to follow root development. Root surface permeability tests and the biochemical quantification of lignin were performed to complement the structural data.Key ResultsThe data presented suggest a redefinition of lrt1 function in lateral roots as a promoter of later development; however, neither the complete absence of lateral roots nor the frequency of their initiation is linked to lrt1 function. The developmental effects of lrt1 are under strong environmental influences. Mutant primordia are affected in structure, growth and emergence; and the majority of primordia terminate their growth during this last step, or shortly thereafter. The lateral roots are impaired in the maintenance of the root apical meristem. The primary root shows disturbances in the organization of both epidermal and subepidermal layers. The lrt1-related cell-wall modifications include: lignification in peripheral layers, the deposition of polyphenolic substances and a higher activity of peroxidase.ConclusionsThe present study provides novel insights into the function of the lrt1 gene in root system development. The lrt1 gene participates in the spatial distribution of initiation, but not in its frequency. Later, the development of lateral roots is strongly affected. The effect of the lrt1 mutation is not as obvious in the primary root, with no influences observed on the root apical meristem structure and maintenance; however, development of the epidermis and cortex are impaired.