The Adaptor-Related Protein Complex 2, Alpha 2 Subunit (AP2α2) Gene is a Peroxisome Proliferator-Activated Receptor Cardiac Target Gene

• Published in: Protein Journal 2012, Vol.31 (1), p.75-83
• Main Authors: Buroker, Norman E., Huang, Jie-Yu, Barboza, Julia, Ledee, Dolena R., Eastman, Rocky J., Reinecke, Hans, Ning, Xue-Han, Bassuk, James A., Portman, Michael A.
• Format: Article
• Language: English
• Subjects: Adaptor Protein Complex 2 - genetics; Adaptor Protein Complex 2 - metabolism; Adaptor Protein Complex alpha Subunits - genetics; Adaptor Protein Complex alpha Subunits - metabolism; Animal Anatomy; Animals; Biochemistry; Bioorganic Chemistry; Cardiovascular system; Cell differentiation; Cell Line; Chemistry; Chemistry and Materials Science; Differentiation; Gene expression; Gene Expression Regulation; Genes; Genetic engineering; Genetic transcription; Heart; Histology; Humans; Lipid metabolism; Lipids; Mice; Mice, Transgenic; Morphology; Myocardium - metabolism; Organic Chemistry; Peroxisome proliferator-activated receptors; Plasma membranes; Polymerase chain reaction; PPAR alpha - genetics; PPAR alpha - metabolism; Promoter Regions, Genetic; Promoters; Protein transport; Proteins; Regulatory sequences; Response Elements; Retinoid X receptors; Rodents; Steroid hormones; Thyroid; Thyroid hormone receptors; Transcription; Transcription activation; Transcription factors; Transgenic animals; Transgenic mice; Up-Regulation
• ISSN: 1572-3887; 1573-4943; 1875-8355
• DOI: 10.1007/s10930-011-9379-0

A peroxisome proliferator-actived receptor (PPAR) response element (RE) in the promoter region of the adaptor-related protein complex 2, alpha 2 subunit (AP2α2) of mouse heart has been identified. The steroid hormone nuclear PPARs and the retinoid X receptors (RXRs) are important transcriptional factors that regulate gene expression, cell differentiation and lipid metabolism. They form homo- (RXR) and hetero- (PPAR-RXR) dimers that bind DNA at various REs. The AP2α2 gene is part of complex and process that transports lipids and proteins from the plasma membrane to the endosomal system. A PPAR activator (Wy14643) and DMSO (vehicle) was introduced into control and δ337T thyroid hormone receptor (TRβ1) transgenic mice. Heart tissue was extracted and AP2α2 gene expression was compared using Affymetrix expression arrays and qRT PCR among four groups [control, control with Wy14643, δ337T TRβ1 and δ337T TRβ1 with Wy14643]. The gene expression of AP2α2 in the Wy14643 control and transgenic mouse groups was significantly up regulated over the vehicle mouse groups in both the array ( p