Single-File Diffusion of Flagellin in Flagellar Filaments

A bacterial flagellar filament is a cylindrical crystal of a protein known as flagellin. Flagellin subunits travel from the cytoplasm through a 2 nm axial pore and polymerize at the filament’s distal end. They are supplied by a pump in the cell membrane powered by a proton-motive force. In a recent...

Full description

Saved in:
Bibliographic Details
Published in:Biophysical journal 2013-07, Vol.105 (1), p.182-184
Main Authors: Stern, Alan S., Berg, Howard C.
Format: Article
Language:English
Subjects:
Bacteria
cell membranes
crystal proteins
Cytoplasm
Diffusion
Flagella - metabolism
flagellin
Flagellin - chemistry
Flagellin - metabolism
Models, Molecular
Molecular Machines, Motors, and Nanoscale Biophysics
polymerization
Protein Structure, Secondary
Proteins
Protons
Simulation
travel
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c503t-cd5309cfc3e1acd3b1572bda1a4d573d8e47ae1d744509244aa3c7a936c0ee83
cites cdi_FETCH-LOGICAL-c503t-cd5309cfc3e1acd3b1572bda1a4d573d8e47ae1d744509244aa3c7a936c0ee83
container_end_page 184
container_issue 1
container_start_page 182
container_title Biophysical journal
container_volume 105
creator Stern, Alan S.
Berg, Howard C.
description A bacterial flagellar filament is a cylindrical crystal of a protein known as flagellin. Flagellin subunits travel from the cytoplasm through a 2 nm axial pore and polymerize at the filament’s distal end. They are supplied by a pump in the cell membrane powered by a proton-motive force. In a recent experiment, it was observed that growth proceeded at a rate of approximately one subunit every 2 s. Here, we asked whether transport of subunits through the pore at this rate could be effected by single-file diffusion, which we simulated by a random walk on a one-dimensional lattice. Assuming that the subunits are α-helical, the answer is yes, by a comfortable margin.
doi_str_mv 10.1016/j.bpj.2013.05.030
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3699742</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006349513006139</els_id><sourcerecordid>3020619831</sourcerecordid><originalsourceid>FETCH-LOGICAL-c503t-cd5309cfc3e1acd3b1572bda1a4d573d8e47ae1d744509244aa3c7a936c0ee83</originalsourceid><addsrcrecordid>eNp9kV2L1DAUhoMo7rj6A7zRwt5403ry1bQIgqyOKyx4sev1IZOejimdZkymC_77TZlxUS8WAiHkOW_OycPYaw4VB16_H6rNfqgEcFmBrkDCE7biWokSoKmfshUA1KVUrT5jL1IaALjQwJ-zMyEbIYU0K9be-Gk7Urn2IxWffd_PyYepCH2xHu2WxtFPRV6ng41FBu2OpkN6yZ71dkz06rSfs9v1l9vLq_L6-9dvl5-uS6dBHkrXaQmt650kbl0nN1wbsekst6rTRnYNKWOJd0YpDa1QylrpjG1l7YCokefs4zF2P2921Ln8dLQj7qPf2fgbg_X4783kf-I23KGs29YokQPenQJi-DVTOuDOJ7cMM1GYE3LZNkoIreqMXvyHDmGOU54OuQIQtVFmCeRHysWQUqT-oRkOuHjBAbMXXLwgaMxecs2bv6d4qPgjIgNvj0BvA9pt9Al_3OQEnaVxyWGJ-HAkKH_2naeIyXmaHHU-kjtgF_wjDdwDDBmmOw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1400267472</pqid></control><display><type>article</type><title>Single-File Diffusion of Flagellin in Flagellar Filaments</title><source>PubMed Central</source><creator>Stern, Alan S. ; Berg, Howard C.</creator><creatorcontrib>Stern, Alan S. ; Berg, Howard C.</creatorcontrib><description>A bacterial flagellar filament is a cylindrical crystal of a protein known as flagellin. Flagellin subunits travel from the cytoplasm through a 2 nm axial pore and polymerize at the filament’s distal end. They are supplied by a pump in the cell membrane powered by a proton-motive force. In a recent experiment, it was observed that growth proceeded at a rate of approximately one subunit every 2 s. Here, we asked whether transport of subunits through the pore at this rate could be effected by single-file diffusion, which we simulated by a random walk on a one-dimensional lattice. Assuming that the subunits are α-helical, the answer is yes, by a comfortable margin.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1016/j.bpj.2013.05.030</identifier><identifier>PMID: 23823237</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Bacteria ; cell membranes ; crystal proteins ; Cytoplasm ; Diffusion ; Flagella - metabolism ; flagellin ; Flagellin - chemistry ; Flagellin - metabolism ; Models, Molecular ; Molecular Machines, Motors, and Nanoscale Biophysics ; polymerization ; Protein Structure, Secondary ; Proteins ; Protons ; Simulation ; travel</subject><ispartof>Biophysical journal, 2013-07, Vol.105 (1), p.182-184</ispartof><rights>2013 Biophysical Society</rights><rights>Copyright © 2013 Biophysical Society. Published by Elsevier Inc. All rights reserved.</rights><rights>Copyright Biophysical Society Jul 2, 2013</rights><rights>2013 by the Biophysical Society. 2013 Biophysical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-cd5309cfc3e1acd3b1572bda1a4d573d8e47ae1d744509244aa3c7a936c0ee83</citedby><cites>FETCH-LOGICAL-c503t-cd5309cfc3e1acd3b1572bda1a4d573d8e47ae1d744509244aa3c7a936c0ee83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3699742/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3699742/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23823237$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stern, Alan S.</creatorcontrib><creatorcontrib>Berg, Howard C.</creatorcontrib><title>Single-File Diffusion of Flagellin in Flagellar Filaments</title><title>Biophysical journal</title><addtitle>Biophys J</addtitle><description>A bacterial flagellar filament is a cylindrical crystal of a protein known as flagellin. Flagellin subunits travel from the cytoplasm through a 2 nm axial pore and polymerize at the filament’s distal end. They are supplied by a pump in the cell membrane powered by a proton-motive force. In a recent experiment, it was observed that growth proceeded at a rate of approximately one subunit every 2 s. Here, we asked whether transport of subunits through the pore at this rate could be effected by single-file diffusion, which we simulated by a random walk on a one-dimensional lattice. Assuming that the subunits are α-helical, the answer is yes, by a comfortable margin.</description><subject>Bacteria</subject><subject>cell membranes</subject><subject>crystal proteins</subject><subject>Cytoplasm</subject><subject>Diffusion</subject><subject>Flagella - metabolism</subject><subject>flagellin</subject><subject>Flagellin - chemistry</subject><subject>Flagellin - metabolism</subject><subject>Models, Molecular</subject><subject>Molecular Machines, Motors, and Nanoscale Biophysics</subject><subject>polymerization</subject><subject>Protein Structure, Secondary</subject><subject>Proteins</subject><subject>Protons</subject><subject>Simulation</subject><subject>travel</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9kV2L1DAUhoMo7rj6A7zRwt5403ry1bQIgqyOKyx4sev1IZOejimdZkymC_77TZlxUS8WAiHkOW_OycPYaw4VB16_H6rNfqgEcFmBrkDCE7biWokSoKmfshUA1KVUrT5jL1IaALjQwJ-zMyEbIYU0K9be-Gk7Urn2IxWffd_PyYepCH2xHu2WxtFPRV6ng41FBu2OpkN6yZ71dkz06rSfs9v1l9vLq_L6-9dvl5-uS6dBHkrXaQmt650kbl0nN1wbsekst6rTRnYNKWOJd0YpDa1QylrpjG1l7YCokefs4zF2P2921Ln8dLQj7qPf2fgbg_X4783kf-I23KGs29YokQPenQJi-DVTOuDOJ7cMM1GYE3LZNkoIreqMXvyHDmGOU54OuQIQtVFmCeRHysWQUqT-oRkOuHjBAbMXXLwgaMxecs2bv6d4qPgjIgNvj0BvA9pt9Al_3OQEnaVxyWGJ-HAkKH_2naeIyXmaHHU-kjtgF_wjDdwDDBmmOw</recordid><startdate>20130702</startdate><enddate>20130702</enddate><creator>Stern, Alan S.</creator><creator>Berg, Howard C.</creator><general>Elsevier Inc</general><general>Biophysical Society</general><general>The Biophysical Society</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130702</creationdate><title>Single-File Diffusion of Flagellin in Flagellar Filaments</title><author>Stern, Alan S. ; Berg, Howard C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-cd5309cfc3e1acd3b1572bda1a4d573d8e47ae1d744509244aa3c7a936c0ee83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Bacteria</topic><topic>cell membranes</topic><topic>crystal proteins</topic><topic>Cytoplasm</topic><topic>Diffusion</topic><topic>Flagella - metabolism</topic><topic>flagellin</topic><topic>Flagellin - chemistry</topic><topic>Flagellin - metabolism</topic><topic>Models, Molecular</topic><topic>Molecular Machines, Motors, and Nanoscale Biophysics</topic><topic>polymerization</topic><topic>Protein Structure, Secondary</topic><topic>Proteins</topic><topic>Protons</topic><topic>Simulation</topic><topic>travel</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stern, Alan S.</creatorcontrib><creatorcontrib>Berg, Howard C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stern, Alan S.</au><au>Berg, Howard C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-File Diffusion of Flagellin in Flagellar Filaments</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>2013-07-02</date><risdate>2013</risdate><volume>105</volume><issue>1</issue><spage>182</spage><epage>184</epage><pages>182-184</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>A bacterial flagellar filament is a cylindrical crystal of a protein known as flagellin. Flagellin subunits travel from the cytoplasm through a 2 nm axial pore and polymerize at the filament’s distal end. They are supplied by a pump in the cell membrane powered by a proton-motive force. In a recent experiment, it was observed that growth proceeded at a rate of approximately one subunit every 2 s. Here, we asked whether transport of subunits through the pore at this rate could be effected by single-file diffusion, which we simulated by a random walk on a one-dimensional lattice. Assuming that the subunits are α-helical, the answer is yes, by a comfortable margin.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23823237</pmid><doi>10.1016/j.bpj.2013.05.030</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0006-3495
ispartof Biophysical journal, 2013-07, Vol.105 (1), p.182-184
issn 0006-3495
1542-0086
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3699742
source PubMed Central
subjects Bacteria
cell membranes
crystal proteins
Cytoplasm
Diffusion
Flagella - metabolism
flagellin
Flagellin - chemistry
Flagellin - metabolism
Models, Molecular
Molecular Machines, Motors, and Nanoscale Biophysics
polymerization
Protein Structure, Secondary
Proteins
Protons
Simulation
travel
title Single-File Diffusion of Flagellin in Flagellar Filaments
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T20%3A57%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Single-File%20Diffusion%20of%20Flagellin%20in%20Flagellar%20Filaments&rft.jtitle=Biophysical%20journal&rft.au=Stern,%20Alan%C2%A0S.&rft.date=2013-07-02&rft.volume=105&rft.issue=1&rft.spage=182&rft.epage=184&rft.pages=182-184&rft.issn=0006-3495&rft.eissn=1542-0086&rft_id=info:doi/10.1016/j.bpj.2013.05.030&rft_dat=%3Cproquest_pubme%3E3020619831%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c503t-cd5309cfc3e1acd3b1572bda1a4d573d8e47ae1d744509244aa3c7a936c0ee83%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1400267472&rft_id=info:pmid/23823237&rfr_iscdi=true