Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients

The aim of the study was to evaluate changes in expression of PBP5 gene associated with immunosuppression. A linear locked nucleic acid (LNA) probe was used to measure resistance gene expression by the Flow-FISH method. Expression of the PBP5 gene measured by Flow-FISH was higher in enterococcal str...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2013-01, Vol.2013 (2013), p.1-4
Main Authors: Gołębiewska, J., Rutkowski, Bolesław, Witkowski, Jacek M., Daca, A., Jarzembowski, Tomasz, Dębska-Ślizień, Alicja
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biofilms - drug effects
Cyclosporine - pharmacology
Enterococcus - drug effects
Enterococcus - genetics
Enterococcus - isolation & purification
Gene Expression Regulation, Bacterial - drug effects
Humans
In Situ Hybridization, Fluorescence
Kidney Transplantation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of the study was to evaluate changes in expression of PBP5 gene associated with immunosuppression. A linear locked nucleic acid (LNA) probe was used to measure resistance gene expression by the Flow-FISH method. Expression of the PBP5 gene measured by Flow-FISH was higher in enterococcal strains isolated from renal transplantation (RTx) recipients than in commensal strains. Additionally, in contrast to commensal strains in isolates from RTx patients, PBP5 gene expression was 17.45% higher in biofilms than in planktonic cells. Detailed comparison also showed that cyclosporine seemed to induce higher expression of PBP5 as compared to tacrolimus.
ISSN:2314-6133
2314-6141
DOI:10.1155/2013/687156