Adult Human Gingival Epithelial Cells as a Source for Whole-tooth Bioengineering

Teeth develop from interactions between embryonic oral epithelium and neural-crest-derived mesenchyme. These cells can be separated into single-cell populations and recombined to form normal teeth, providing a basis for bioengineering new teeth if suitable, non-embryonic cell sources can be identifi...

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Bibliographic Details
Published in:Journal of dental research 2013-04, Vol.92 (4), p.329-334
Main Authors: Angelova Volponi, A., Kawasaki, M., Sharpe, P.T.
Format: Article
Language:English
Subjects:
Animals
Bioengineering
Bone marrow
Cell Communication - physiology
Cell culture
Cell Differentiation
Coculture Techniques
Dental enamel
Dental roots
Dentin
Dentistry
Enamel
Epithelial cells
Epithelial Cells - cytology
Epithelial Cells - physiology
Epithelium
Gingiva - cytology
Humans
Kidneys
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - physiology
Mesenchyme
Mice
Mice, SCID
Odontogenesis - physiology
Penicillin
Research Reports
Studies
Surgery
Teeth
Tissue engineering
Tissue Engineering - methods
Tooth - cytology
Tooth - embryology
Tooth - growth & development
Tooth Germ - cytology
Tooth Germ - physiology
Transplants & implants
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Summary:Teeth develop from interactions between embryonic oral epithelium and neural-crest-derived mesenchyme. These cells can be separated into single-cell populations and recombined to form normal teeth, providing a basis for bioengineering new teeth if suitable, non-embryonic cell sources can be identified. We show here that cells can be isolated from adult human gingival tissue that can be expanded in vitro and, when combined with mouse embryonic tooth mesenchyme cells, form teeth. Teeth with developing roots can be produced from this cell combination following transplantation into renal capsules. These bioengineered teeth contain dentin and enamel with ameloblast-like cells and rests of Malassez of human origin.
ISSN:0022-0345
1544-0591
1544-0591
DOI:10.1177/0022034513481041