Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells

• Published in: Stem cell research & therapy 2013-03, Vol.4 (2), p.27-27, Article 27
• Main Authors: Patrikoski, Mimmi, Juntunen, Miia, Boucher, Shayne, Campbell, Andrew, Vemuri, Mohan C, Mannerström, Bettina, Miettinen, Susanna
• Format: Article
• Language: English
• Subjects: Adipose Tissue - cytology; Adult; Antigens, CD - genetics; Antigens, CD - metabolism; Cell Culture Techniques - methods; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cell- and Tissue-Based Therapy; Cells, Cultured; Cellular therapy; Chemical tests and reagents; Chondrogenesis - drug effects; Coatings; Culture Media, Serum-Free - pharmacology; Female; Health aspects; Humans; Immunophenotyping; Medical research; Medicine, Experimental; Middle Aged; Osteogenesis - drug effects; Stem Cell Transplantation; Stem cells; Stem Cells - cytology; Stem Cells - metabolism; Technology application; Transplantation; Transplantation of organs, tissues, etc
• ISSN: 1757-6512; 1757-6512
• DOI: 10.1186/scrt175

Adipose tissue is an attractive and abundant source of multipotent stem cells. Human adipose stem cells (ASCs) have shown to have therapeutic relevancy in diverse clinical applications. Nevertheless, expansion of ASCs is often necessary before performing clinical studies. Standard in vitro cell-culture techniques use animal-derived reagents that should be avoided in clinical use because of safety issues. Therefore, xeno- and serum-free (XF/SF) reagents are highly desirable for enhancing the safety and quality of the transplanted ASCs. In the current study, animal component-free isolation and cell-expansion protocols were developed for ASCs. StemPro MSC SFM XF medium with either CELLstart™ CTS™ coating or Coating Matrix Kit were tested for their ability to support XF/SF growth. Basic stem-cell characteristics such as immunophenotype (CD3, CD11a, CD14, CD19, CD34, CD45RO, CD54, CD73, CD80, CD86, CD90, CD105, HLA-DR), proliferation, and differentiation potential were assessed in XF/SF conditions and compared with human serum (HS) or traditionally used fetal bovine serum (FBS) cultures. ASCs cultured in XF/SF conditions had significantly higher proliferation rates compared with HS/FBS cultures. Characteristic immunophenotypes of ASCs were maintained in every condition; however, cells expanded in XF/SF conditions showed significantly lower expression of CD54 (intercellular adhesion molecule 1, ICAM-1) at low passage number. Further, multilineage differentiation potential of ASCs was maintained in every culture condition. Our findings demonstrated that the novel XF/SF conditions maintained the basic stem cell features of ASCs and the animal-free workflow followed in this study has great potential in clinical cell therapies.