Reduction of methylviologen-mediated oxidative stress tolerance in antisense transgenic tobacco seedlings through restricted expression of StAPX

Ascorbate peroxidases are directly involved in reactive oxygen species (ROS) scavenging by reducing hydrogen peroxide to water. The tomato thylakoid-bound ascorbate peroxidase gene ( StAPX ) was introduced into tobacco. RNA gel blot analysis confirmed that StAPX in tomato leaves was induced by methy...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2013-07, Vol.14 (7), p.578-585
Main Authors: Sun, Wei-hong, Wang, Yong, He, Hua-gang, Li, Xue, Song, Wan, Du, Bin, Meng, Qing-wei
Format: Article
Language:English
Subjects:
Antioxidants - chemistry
Antisense
Ascorbate Peroxidases - biosynthesis
Ascorbate Peroxidases - genetics
Biomedical and Life Sciences
Biomedicine
Biotechnology
Chlorophyll - chemistry
Chloroplasts - genetics
Electric Conductivity
Gene Expression Regulation, Plant
Herbicides - chemistry
Lycopersicon esculentum
Malondialdehyde - chemistry
Nicotiana - genetics
Nicotiana - physiology
Oligonucleotides, Antisense - genetics
Oxidative Stress
Paraquat - chemistry
Phenotype
Plants, Genetically Modified - genetics
Seedlings - genetics
Solanum lycopersicum - enzymology
Stress, Physiological
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ascorbate peroxidases are directly involved in reactive oxygen species (ROS) scavenging by reducing hydrogen peroxide to water. The tomato thylakoid-bound ascorbate peroxidase gene ( StAPX ) was introduced into tobacco. RNA gel blot analysis confirmed that StAPX in tomato leaves was induced by methylviologen-mediated oxidative stress. The sense transgenic seedlings exhibited higher tAPX activity than that of the wild type (WT) plants under oxidative stress conditions, while the antisense seedlings exhibited lower tAPX activity. Lower APX activities of antisense transgenic seedlings caused higher malondialdehyde contents and relative electrical conductivity. The sense transgenic seedlings with higher tAPX activity maintained higher chlorophyll content and showed the importance of tAPX in maintaining the optimal chloroplast development under methylviologen stress conditions, whereas the antisense lines maintained lower chlorophyll content than WT seedlings. Results indicated that the over-expression of StAPX enhanced tolerance to methylviologen-mediated oxidative stress in sense transgenic tobacco early seedlings, whereas the suppression of StAPX in antisense transgenic seedlings showed high sensitivity to oxidative stress.
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B1200190