Mimicking cellular transport mechanism in stem cells through endosomal escape of new peptide-coated quantum dots

Protein transport is an important phenomenon in biological systems. Proteins are transported via several mechanisms to reach their destined compartment of cell for its complete function. One such mechanism is the microtubule mediated protein transport. Up to now, there are no reports on synthetic sy...

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Bibliographic Details
Published in:Scientific reports 2013-07, Vol.3 (1), p.2184, Article 2184
Main Authors: Narayanan, Karthikeyan, Yen, Swee Kuan, Dou, Qingqing, Padmanabhan, Parasuraman, Sudhaharan, Thankiah, Ahmed, Sohail, Ying, Jackie Y., Selvan, Subramanian Tamil
Format: Article
Language:English
Subjects:
631/532/2118/2074
631/61/350/354
631/80/2373
639/301/357/1017
Amino Acid Sequence
Biological Transport
Cell Nucleus - metabolism
Drug delivery
Endosomes - metabolism
Humanities and Social Sciences
Humans
Localization
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Mesenchyme
Microscopy, Confocal
Microtubules
Microtubules - metabolism
Mimicry
Molecular Sequence Data
multidisciplinary
Nuclear Localization Signals
Peptides
Peptides - chemistry
Peptides - metabolism
Protein Binding
Protein transport
Proteins
Quantum dots
Quantum Dots - chemistry
Science
Solubility
Stem cells
Streptavidin
Therapeutic applications
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Summary:Protein transport is an important phenomenon in biological systems. Proteins are transported via several mechanisms to reach their destined compartment of cell for its complete function. One such mechanism is the microtubule mediated protein transport. Up to now, there are no reports on synthetic systems mimicking the biological protein transport mechanism. Here we report a highly efficient method of mimicking the microtubule mediated protein transport using newly designed biotinylated peptides encompassing a microtubule-associated sequence (MTAS) and a nuclear localization signaling (NLS) sequence and their final conjugation with streptavidin-coated CdSe/ZnS quantum dots (QDs). Our results demonstrate that these novel bio-conjugated QDs enhance the endosomal escape and promote targeted delivery into the nucleus of human mesenchymal stem cells via microtubules. Mimicking the cellular transport mechanism in stem cells is highly desirable for diagnostics, targeting and therapeutic applications, opening up new avenues in the area of drug delivery.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep02184