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Mimicking cellular transport mechanism in stem cells through endosomal escape of new peptide-coated quantum dots
Protein transport is an important phenomenon in biological systems. Proteins are transported via several mechanisms to reach their destined compartment of cell for its complete function. One such mechanism is the microtubule mediated protein transport. Up to now, there are no reports on synthetic sy...
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| Published in: | Scientific reports 2013-07, Vol.3 (1), p.2184, Article 2184 |
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| Main Authors: | , , , , , , , |
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| creator | Narayanan, Karthikeyan Yen, Swee Kuan Dou, Qingqing Padmanabhan, Parasuraman Sudhaharan, Thankiah Ahmed, Sohail Ying, Jackie Y. Selvan, Subramanian Tamil |
| description | Protein transport is an important phenomenon in biological systems. Proteins are transported via several mechanisms to reach their destined compartment of cell for its complete function. One such mechanism is the microtubule mediated protein transport. Up to now, there are no reports on synthetic systems mimicking the biological protein transport mechanism. Here we report a highly efficient method of mimicking the microtubule mediated protein transport using newly designed biotinylated peptides encompassing a microtubule-associated sequence (MTAS) and a nuclear localization signaling (NLS) sequence and their final conjugation with streptavidin-coated CdSe/ZnS quantum dots (QDs). Our results demonstrate that these novel bio-conjugated QDs enhance the endosomal escape and promote targeted delivery into the nucleus of human mesenchymal stem cells via microtubules. Mimicking the cellular transport mechanism in stem cells is highly desirable for diagnostics, targeting and therapeutic applications, opening up new avenues in the area of drug delivery. |
| doi_str_mv | 10.1038/srep02184 |
| format | article |
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Proteins are transported via several mechanisms to reach their destined compartment of cell for its complete function. One such mechanism is the microtubule mediated protein transport. Up to now, there are no reports on synthetic systems mimicking the biological protein transport mechanism. Here we report a highly efficient method of mimicking the microtubule mediated protein transport using newly designed biotinylated peptides encompassing a microtubule-associated sequence (MTAS) and a nuclear localization signaling (NLS) sequence and their final conjugation with streptavidin-coated CdSe/ZnS quantum dots (QDs). Our results demonstrate that these novel bio-conjugated QDs enhance the endosomal escape and promote targeted delivery into the nucleus of human mesenchymal stem cells via microtubules. Mimicking the cellular transport mechanism in stem cells is highly desirable for diagnostics, targeting and therapeutic applications, opening up new avenues in the area of drug delivery.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep02184</identifier><identifier>PMID: 23851637</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/532/2118/2074 ; 631/61/350/354 ; 631/80/2373 ; 639/301/357/1017 ; Amino Acid Sequence ; Biological Transport ; Cell Nucleus - metabolism ; Drug delivery ; Endosomes - metabolism ; Humanities and Social Sciences ; Humans ; Localization ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Mesenchyme ; Microscopy, Confocal ; Microtubules ; Microtubules - metabolism ; Mimicry ; Molecular Sequence Data ; multidisciplinary ; Nuclear Localization Signals ; Peptides ; Peptides - chemistry ; Peptides - metabolism ; Protein Binding ; Protein transport ; Proteins ; Quantum dots ; Quantum Dots - chemistry ; Science ; Solubility ; Stem cells ; Streptavidin ; Therapeutic applications</subject><ispartof>Scientific reports, 2013-07, Vol.3 (1), p.2184, Article 2184</ispartof><rights>The Author(s) 2013</rights><rights>Copyright Nature Publishing Group Jul 2013</rights><rights>Copyright © 2013, Macmillan Publishers Limited. All rights reserved 2013 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-4c43843b18fdaebc5d0d13aa0d09598ae8623ae41bc01547ec9b483f41d525543</citedby><cites>FETCH-LOGICAL-c504t-4c43843b18fdaebc5d0d13aa0d09598ae8623ae41bc01547ec9b483f41d525543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1897435067/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1897435067?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23851637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Narayanan, Karthikeyan</creatorcontrib><creatorcontrib>Yen, Swee Kuan</creatorcontrib><creatorcontrib>Dou, Qingqing</creatorcontrib><creatorcontrib>Padmanabhan, Parasuraman</creatorcontrib><creatorcontrib>Sudhaharan, Thankiah</creatorcontrib><creatorcontrib>Ahmed, Sohail</creatorcontrib><creatorcontrib>Ying, Jackie Y.</creatorcontrib><creatorcontrib>Selvan, Subramanian Tamil</creatorcontrib><title>Mimicking cellular transport mechanism in stem cells through endosomal escape of new peptide-coated quantum dots</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Protein transport is an important phenomenon in biological systems. Proteins are transported via several mechanisms to reach their destined compartment of cell for its complete function. One such mechanism is the microtubule mediated protein transport. Up to now, there are no reports on synthetic systems mimicking the biological protein transport mechanism. Here we report a highly efficient method of mimicking the microtubule mediated protein transport using newly designed biotinylated peptides encompassing a microtubule-associated sequence (MTAS) and a nuclear localization signaling (NLS) sequence and their final conjugation with streptavidin-coated CdSe/ZnS quantum dots (QDs). Our results demonstrate that these novel bio-conjugated QDs enhance the endosomal escape and promote targeted delivery into the nucleus of human mesenchymal stem cells via microtubules. Mimicking the cellular transport mechanism in stem cells is highly desirable for diagnostics, targeting and therapeutic applications, opening up new avenues in the area of drug delivery.</description><subject>631/532/2118/2074</subject><subject>631/61/350/354</subject><subject>631/80/2373</subject><subject>639/301/357/1017</subject><subject>Amino Acid Sequence</subject><subject>Biological Transport</subject><subject>Cell Nucleus - metabolism</subject><subject>Drug delivery</subject><subject>Endosomes - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Localization</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mesenchyme</subject><subject>Microscopy, Confocal</subject><subject>Microtubules</subject><subject>Microtubules - metabolism</subject><subject>Mimicry</subject><subject>Molecular Sequence Data</subject><subject>multidisciplinary</subject><subject>Nuclear Localization Signals</subject><subject>Peptides</subject><subject>Peptides - chemistry</subject><subject>Peptides - metabolism</subject><subject>Protein Binding</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>Quantum dots</subject><subject>Quantum Dots - chemistry</subject><subject>Science</subject><subject>Solubility</subject><subject>Stem cells</subject><subject>Streptavidin</subject><subject>Therapeutic applications</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNplkV9LHDEUxYMoVXQf-gVKoE8KU_N3J_NSEKltQfFFn0MmubOb7UwyJpmWfvuOXV1WvC_3wv1x7rkchD5S8oUSri5zgpEwqsQBOmFEyIpxxg735mO0yHlD5pKsEbT5gI4ZV5IueX2Cxjs_ePvLhxW20PdTbxIuyYQ8xlTwAHZtgs8D9gHnAsN_KOOyTnFarTEEF3McTI8hWzMCjh0O8AePMBbvoLLRFHD4aTKhTAN2seQzdNSZPsPipZ-ix5tvD9c_qtv77z-vr24rK4kolbCCK8FbqjpnoLXSEUe5McSRRjbKgFoybkDQ1hIqRQ22aYXinaBOMikFP0Vft7rj1A7gLIT5rV6PyQ8m_dXReP12E_xar-JvzWtKiahngc8vAik-TZCL3sQphdmzpqqpBZdk-UydbymbYp6j6HYXKNHP-ehdPjP7ad_SjnxNYwYutkCeV2EFae_kO7V_XGGcgw</recordid><startdate>20130715</startdate><enddate>20130715</enddate><creator>Narayanan, Karthikeyan</creator><creator>Yen, Swee Kuan</creator><creator>Dou, Qingqing</creator><creator>Padmanabhan, Parasuraman</creator><creator>Sudhaharan, Thankiah</creator><creator>Ahmed, Sohail</creator><creator>Ying, Jackie Y.</creator><creator>Selvan, Subramanian Tamil</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20130715</creationdate><title>Mimicking cellular transport mechanism in stem cells through endosomal escape of new peptide-coated quantum dots</title><author>Narayanan, Karthikeyan ; Yen, Swee Kuan ; Dou, Qingqing ; Padmanabhan, Parasuraman ; Sudhaharan, Thankiah ; Ahmed, Sohail ; Ying, Jackie Y. ; Selvan, Subramanian Tamil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-4c43843b18fdaebc5d0d13aa0d09598ae8623ae41bc01547ec9b483f41d525543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>631/532/2118/2074</topic><topic>631/61/350/354</topic><topic>631/80/2373</topic><topic>639/301/357/1017</topic><topic>Amino Acid Sequence</topic><topic>Biological Transport</topic><topic>Cell Nucleus - metabolism</topic><topic>Drug delivery</topic><topic>Endosomes - metabolism</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Localization</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mesenchyme</topic><topic>Microscopy, Confocal</topic><topic>Microtubules</topic><topic>Microtubules - metabolism</topic><topic>Mimicry</topic><topic>Molecular Sequence Data</topic><topic>multidisciplinary</topic><topic>Nuclear Localization Signals</topic><topic>Peptides</topic><topic>Peptides - chemistry</topic><topic>Peptides - metabolism</topic><topic>Protein Binding</topic><topic>Protein transport</topic><topic>Proteins</topic><topic>Quantum dots</topic><topic>Quantum Dots - chemistry</topic><topic>Science</topic><topic>Solubility</topic><topic>Stem cells</topic><topic>Streptavidin</topic><topic>Therapeutic applications</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Narayanan, Karthikeyan</creatorcontrib><creatorcontrib>Yen, Swee Kuan</creatorcontrib><creatorcontrib>Dou, Qingqing</creatorcontrib><creatorcontrib>Padmanabhan, Parasuraman</creatorcontrib><creatorcontrib>Sudhaharan, Thankiah</creatorcontrib><creatorcontrib>Ahmed, Sohail</creatorcontrib><creatorcontrib>Ying, Jackie Y.</creatorcontrib><creatorcontrib>Selvan, Subramanian Tamil</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Narayanan, Karthikeyan</au><au>Yen, Swee Kuan</au><au>Dou, Qingqing</au><au>Padmanabhan, Parasuraman</au><au>Sudhaharan, Thankiah</au><au>Ahmed, Sohail</au><au>Ying, Jackie Y.</au><au>Selvan, Subramanian Tamil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mimicking cellular transport mechanism in stem cells through endosomal escape of new peptide-coated quantum dots</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2013-07-15</date><risdate>2013</risdate><volume>3</volume><issue>1</issue><spage>2184</spage><pages>2184-</pages><artnum>2184</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Protein transport is an important phenomenon in biological systems. Proteins are transported via several mechanisms to reach their destined compartment of cell for its complete function. One such mechanism is the microtubule mediated protein transport. Up to now, there are no reports on synthetic systems mimicking the biological protein transport mechanism. Here we report a highly efficient method of mimicking the microtubule mediated protein transport using newly designed biotinylated peptides encompassing a microtubule-associated sequence (MTAS) and a nuclear localization signaling (NLS) sequence and their final conjugation with streptavidin-coated CdSe/ZnS quantum dots (QDs). Our results demonstrate that these novel bio-conjugated QDs enhance the endosomal escape and promote targeted delivery into the nucleus of human mesenchymal stem cells via microtubules. Mimicking the cellular transport mechanism in stem cells is highly desirable for diagnostics, targeting and therapeutic applications, opening up new avenues in the area of drug delivery.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>23851637</pmid><doi>10.1038/srep02184</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 631/532/2118/2074 631/61/350/354 631/80/2373 639/301/357/1017 Amino Acid Sequence Biological Transport Cell Nucleus - metabolism Drug delivery Endosomes - metabolism Humanities and Social Sciences Humans Localization Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Mesenchyme Microscopy, Confocal Microtubules Microtubules - metabolism Mimicry Molecular Sequence Data multidisciplinary Nuclear Localization Signals Peptides Peptides - chemistry Peptides - metabolism Protein Binding Protein transport Proteins Quantum dots Quantum Dots - chemistry Science Solubility Stem cells Streptavidin Therapeutic applications |
| title | Mimicking cellular transport mechanism in stem cells through endosomal escape of new peptide-coated quantum dots |
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