Kisspeptin Activation of TRPC4 Channels in Female GnRH Neurons Requires PIP2 Depletion and cSrc Kinase Activation

• Published in: Endocrinology (Philadelphia) 2013-08, Vol.154 (8), p.2772-2783
• Main Authors: Zhang, Chunguang, Bosch, Martha A, Rønnekleiv, Oline K, Kelly, Martin J
• Format: Article
• Language: English
• Subjects: 1-Phosphatidylinositol 4-kinase; Androstadienes - pharmacology; Animals; Biological and medical sciences; Calcium (extracellular); Calcium (intracellular); Calcium - metabolism; Calcium buffering; Calcium channels; Calcium ions; Cell activation; Channels; CSK Tyrosine-Protein Kinase; Depletion; Dialysis; Enzyme Activation - drug effects; Excitability; Female; Fundamental and applied biological sciences. Psychology; Genistein; Genistein - pharmacology; Gonadotropin-releasing hormone; Gonadotropin-Releasing Hormone - genetics; Gonadotropin-Releasing Hormone - metabolism; Gonadotropins; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Inositol trisphosphate; Inositols; Intracellular; Intracellular signalling; Kinases; Kiss1 protein; Kisspeptins - pharmacology; Membrane Potentials - drug effects; Mice; Mice, Transgenic; mRNA; Neuroendocrinology; Neurons; Neurons - cytology; Neurons - metabolism; Neurons - physiology; Ovariectomy; Patch-Clamp Techniques; Phosphatidylinositol 3-Kinases - metabolism; Phosphatidylinositol 4,5-diphosphate; Phosphatidylinositol 4,5-Diphosphate - metabolism; Phosphoinositide-3 Kinase Inhibitors; Phospholipase C; Pituitary; Pituitary (anterior); Protein kinase C; Protein Kinase C - antagonists & inhibitors; Protein Kinase C - metabolism; Protein Kinase Inhibitors - pharmacology; Proteins; Pyrimidines - pharmacology; Receptors; Signal transduction; src-Family Kinases - antagonists & inhibitors; src-Family Kinases - metabolism; Thapsigargin; Transient receptor potential proteins; TRPC Cation Channels - genetics; TRPC Cation Channels - metabolism; Tyrosine; Vertebrates: endocrinology; Wortmannin
• ISSN: 0013-7227; 1945-7170
• DOI: 10.1210/en.2013-1180

Kisspeptin signaling via its Gαq-coupled receptor GPR54 plays a crucial role in modulating GnRH neuronal excitability, which controls pituitary gonadotropins secretion and ultimately reproduction. Kisspeptin potently depolarizes GnRH neurons primarily through the activation of canonical transient receptor potential (TRPC) channels, but the intracellular signaling cascade has not been elucidated. Presently, we have established that kisspeptin activation of TRPC channels requires multiple membrane and intracellular signaling molecules. First, phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis by phospholipase Cβ is required because whole-cell dialysis of Dioctanoylglycerol-PIP2 (DiC8-PIP2) inhibited the kisspeptin activation of TRPC channels, and the phosphatidylinositol 4-kinase inhibitor wortmannin, which attenuates PIP2 synthesis, prolonged TRPC channel activation. Using single cell RT-PCR, we identified that the mRNA for the PIP2-interacting TRPC channel subunit, TRPC4α, is expressed in GnRH neurons. Depletion of intracellular Ca2+ stores by thapsigargin and inositol 1,4,5-trisphosphate had no effect, indicating that the TRPC channels are not store-operated. Neither removing extracellular Ca2+ nor buffering intracellular Ca2+ with EGTA or BAPTA had any effect on the kisspeptin activation of the TRPC channels. However, the Ca2+ channel blocker Ni2+ inhibited the kisspeptin-induced inward current. Moreover, inhibition of protein kinase C by bisindolylmaleimide-I or calphostin C had no effect, but activation of protein kinase C by phorbol 12,13-dibutyrate occluded the kisspeptin-activated current. Finally, inhibition of the cytoplasmic tyrosine kinase cSrc by genistein or the pyrazolo-pyrimidine PP2 blocked the activation of TRPC channels by kisspeptin. Therefore, TRPC channels in GnRH neurons are receptor-operated, and kisspeptin activates TRPC channels through PIP2 depletion and cSrc tyrosine kinase activation, which is a novel signaling pathway for peptidergic excitation of GnRH neurons.