Leptin Modulates Norepinephrine-Mediated Melatonin Synthesis in Cultured Rat Pineal Gland

Pineal melatonin synthesis can be modulated by many peptides, including insulin. Because melatonin appears to alter leptin synthesis, in this work we aimed to investigate whether leptin would have a role on norepinephrine- (NE-)mediated melatonin synthesis in cultured rat pineal glands. According to...

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Bibliographic Details
Published in:BioMed research international 2013-01, Vol.2013 (2013), p.1-8
Main Authors: Carvalho, Carla Roberta de Oliveira, Cipolla-Neto, Jose, Andrade-Silva, Jéssica, Peliciari-Garcia, Rodrigo Antonio
Format: Article
Language:English
Subjects:
Animals
Aralkylamine N-acetyltransferase
Gene Expression
Intracellular Signaling Peptides and Proteins - metabolism
Leptin
Leptin - administration & dosage
Leptin - metabolism
Melatonin
Melatonin - biosynthesis
Noradrenaline
Norepinephrine - metabolism
Organ Culture Techniques
Phosphorylation
Physiological aspects
Pineal gland
Pineal Gland - metabolism
Rats
Receptors, Leptin - metabolism
Rodents
STAT3 Transcription Factor - metabolism
Studies
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Summary:Pineal melatonin synthesis can be modulated by many peptides, including insulin. Because melatonin appears to alter leptin synthesis, in this work we aimed to investigate whether leptin would have a role on norepinephrine- (NE-)mediated melatonin synthesis in cultured rat pineal glands. According to our data, cultured rat pineal glands express leptin receptor isoform b (Ob-Rb). Pineal expression of Ob-Rb mRNA was also observed in vivo. Administration of leptin (1 nM) associated with NE (1 µM) reduced melatonin content as well as arylalkylamine-N-acetyl transferase (AANAT) activity and expression in cultured pineal glands. Leptin treatment per se induced the expression of STAT3 in cultured pineal glands, but STAT3 does not participate in the leptin modulation of NE-mediated pineal melatonin synthesis. In addition, the expression of inducible cAMP early repressor (ICER) was further induced by leptin challenge when associated with NE. In conclusion, leptin inhibition of pineal melatonin synthesis appears to be mediated by a reduction in AANAT activity and expression as well as by increased expression of Icer mRNA. Peptidergic signaling within the pineal gland appears to be one of the most important signals which modulates melatonin synthesis; leptin, as a member of this system, is not an exception.
ISSN:2314-6133
2314-6141
DOI:10.1155/2013/546516