MicroRNA profiling in the mouse hypothalamus reveals oxytocin‐regulating microRNA

Oxytocin (Oxt), produced in the hypothalamic paraventricular and supraoptic nuclei for transport to and release from the posterior pituitary, was originally discovered through its role in lactation and parturition. Oxt also plays important roles in the central nervous system by influencing various b...

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Bibliographic Details
Published in:Journal of neurochemistry 2013-08, Vol.126 (3), p.331-337
Main Authors: Choi, Ji‐Woong, Kang, Sung‐Min, Lee, Youngkyun, Hong, Su‐Hyung, Sanek, Nicholas A., Young, W. Scott, Lee, Heon‐Jin
Format: Article
Language:English
Subjects:
Animals
AVP
deep sequencing
Enzyme-Linked Immunosorbent Assay
Hormones
hypothalamus
Hypothalamus - metabolism
Male
Mice
Mice, Inbred C57BL
microRNA
MicroRNAs - genetics
MicroRNAs - metabolism
Nervous system
Neurochemistry
OXT
Oxytocin - biosynthesis
Oxytocin - genetics
Peptides
Reverse Transcriptase Polymerase Chain Reaction
RNA Interference - physiology
Transcriptome
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Summary:Oxytocin (Oxt), produced in the hypothalamic paraventricular and supraoptic nuclei for transport to and release from the posterior pituitary, was originally discovered through its role in lactation and parturition. Oxt also plays important roles in the central nervous system by influencing various behaviors. MicroRNAs (miRNAs), endogenous regulators of many genes, are a class of small non‐coding RNAs that mediate post‐transcriptional gene silencing. We performed miRNA expression profiling of the mouse hypothalamus by deep sequencing. Among the sequenced and cross‐mapped small RNAs, expression of known miRNAs and unknown miRNAs candidates were analyzed. We investigated in detail one miRNA, miR‐24, and found that it is a novel regulator of Oxt and controls both transcript and peptide levels of Oxt. These results provide insights into potential neurohypophysial hormone regulation mediated by miRNAs. The mouse hypothalamus miRNAs were profiled by using deep sequencing. Among the miRNAs that are highly expressed in the hypothalamus, we found that miR‐24 regulates oxytocin expression in vitro. This finding expands the potential mechanisms for regulation of hypothalamo‐neurohypophyseal hormones.
ISSN:0022-3042
1471-4159
DOI:10.1111/jnc.12308