Surface modification of PLGA nanoparticles via human serum albumin conjugation for controlled delivery of docetaxel

• Published in: Daru 2013-07, Vol.21 (1), p.58-58, Article 58
• Main Authors: Manoochehri, Saeed, Darvishi, Behrad, Kamalinia, Golnaz, Amini, Mohsen, Fallah, Mahdieh, Ostad, Seyed Naser, Atyabi, Fatemeh, Dinarvand, Rassoul
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biomedicine; Cell Survival - drug effects; Docetaxel; Dosage and administration; Drug delivery systems; Drug targeting; Drugs; Hep G2 Cells; Humans; Lactic Acid - chemistry; Medicinal Chemistry; Nanoparticles; Nanoparticles - chemistry; Particle Size; Pharmaceutical Sciences/Technology; Pharmacology/Toxicology; Polyglycolic Acid - chemistry; Research Article; Serum Albumin - chemistry; Spectroscopy, Fourier Transform Infrared; Taxoids - pharmacology; Vehicles
• ISSN: 1560-8115; 2008-2231; 2008-2231
• DOI: 10.1186/2008-2231-21-58

Background Poly lactic-co-glycolic acid (PLGA) based nanoparticles are considered to be a promising drug carrier in tumor targeting but suffer from the high level of opsonization by reticuloendothelial system due to their hydrophobic structure. As a result surface modification of these nanoparticles has been widely studied as an essential step in their development. Among various surface modifications, human serum albumin (HSA) possesses advantages including small size, hydrophilic surface and accumulation in leaky vasculature of tumors through passive targeting and a probable active transport into tumor tissues. Methods PLGA nanoparticles of docetaxel were prepared by emulsification evaporation method and were surface conjugated with human serum albumin. Fourier transform infrared spectrum was used to confirm the conjugation reaction where nuclear magnetic resonance was utilized for conjugation ratio determination. In addition, transmission electron microscopy showed two different contrast media in conjugated nanoparticles. Furthermore, cytotoxicity of free docetaxel, unconjugated and conjugated PLGA nanoparticles was studied in HepG2 cells. Results Size, zeta potential and drug loading of PLGA nanoparticles were about 199 nm, −11.07 mV, and 4%, respectively where size, zeta potential and drug loading of conjugated nanoparticles were found to be 204 nm, −5.6 mV and 3.6% respectively. Conjugated nanoparticles represented a three-phasic release pattern with a 20% burst effect for docetaxel on the first day. Cytotoxicity experiment showed that the IC50 of HSA conjugated PLGA nanoparticles (5.4 μg) was significantly lower than both free docetaxel (20.2 μg) and unconjugated PLGA nanoparticles (6.2 μg). Conclusion In conclusion surface modification of PLGA nanoparticles through HSA conjugation results in more cytotoxicity against tumor cell lines compared with free docetaxel and unconjugated PLGA nanoparticles. Albumin conjugated PLGA nanoparticles may represent a promising drug delivery system in cancer therapy.