Culture of major pelvic ganglion neurons from adult rat

Successful culturing of neurons from adult animals has been historically difficult for a relatively long time. In this study, we reported the development of a novel method for the isolation and the culture of major pelvic ganglion (MPG) neurons from adult rat. The cultured cells were identified by n...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 2013-08, Vol.65 (4), p.663-669
Main Authors: Cheng, Shigang, Yang, Xinghai, Zhang, Yifan, Xiao, Chuanguo
Format: Article
Language:English
Subjects:
Alzheimer's disease
Antibodies
Biochemistry
Biomedicine
Biotechnology
Cell culture
Chemistry
Chemistry and Materials Science
Cytology
Growth factors
Neurons
Pelvic ganglia
Penicillin
Technical Note
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Summary:Successful culturing of neurons from adult animals has been historically difficult for a relatively long time. In this study, we reported the development of a novel method for the isolation and the culture of major pelvic ganglion (MPG) neurons from adult rat. The cultured cells were identified by neuron morphology and staining with neuronal marker (neurofilament-200, NF-200). The results demonstrate that the new protocol we used was reliable in obtaining a relatively high yield of MPG neurons. Furthermore, it improves the speed and simplicity in neuronal isolation. The viability of neurons can be maintained for about 2 weeks, which should be sufficient for investigating physiological and pathological processes occurring in mature major pelvic ganglia. And this may provide a useful assessment to currently available techniques for the culture of adult neurons.
ISSN:0920-9069
1573-0778
DOI:10.1007/s10616-012-9515-5