Transplantation of endothelial progenitor cells transfected with VEGF165 to restore erectile function in diabetic rats

The present study investigated the effect of transplanting endothelial progenitor cells （EPCs） transfected with the vascular endothelial growth factor gene （VEGF165） into the corpora cavernosa of rats with diabetic erectile dysfunction （ED）. A rat model of diabetic ED was constructed via intraperito...

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Published in:Asian journal of andrology 2011-03, Vol.13 (2), p.332-338
Main Authors: Gou, Xin, He, Wei-Yang, Xiao, Ming-Zhao, Qiu, Ming, Wang, Ming, Deng, Yuan-Zhong, Liu, Chao-Dong, Tang, Zao-Bing, Li, Jie, Chen, Yong
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Diabetes Mellitus, Experimental - complications
Diabetes Mellitus, Experimental - physiopathology
DNA Primers - genetics
Endothelial Cells - physiology
Endothelial Cells - transplantation
Erectile Dysfunction - etiology
Erectile Dysfunction - physiopathology
Erectile Dysfunction - therapy
Male
Neovascularization, Physiologic
Original
Penile Erection - physiology
Penis - blood supply
Rats
Rats, Sprague-Dawley
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Stem Cell Transplantation
Transfection
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - physiology
内皮祖细胞
勃起功能障碍
基因转染
新生血管
糖尿病大鼠
细胞移植
血管内皮生长因子
阴茎海绵体
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container_title Asian journal of andrology
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creator Gou, Xin
He, Wei-Yang
Xiao, Ming-Zhao
Qiu, Ming
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Deng, Yuan-Zhong
Liu, Chao-Dong
Tang, Zao-Bing
Li, Jie
Chen, Yong
description The present study investigated the effect of transplanting endothelial progenitor cells （EPCs） transfected with the vascular endothelial growth factor gene （VEGF165） into the corpora cavernosa of rats with diabetic erectile dysfunction （ED）. A rat model of diabetic ED was constructed via intraperitoneal injection of streptozotocin. After streptozotocin treatment, pre-treated EPCs from each of three groups of rats were transplanted into their corpora cavernosa. Our results, following intracavernosal pressure （ICP） monitoring, showed that ICP increased significantly among rats in the trial group when compared to the results from rats in the blank-plasmid and control groups during basal conditions and electrical stimulation （P〈O.01 for both comparisons）. Histological examination revealed extensive neovascularisation in the corpora cavernosa of rats in the trial group. Fluorescence microscopy indicated that many of the transplanted EPCs in the trial group survived, differentiated into endothelial cells and integrated into the sites of neovascularisation. Based on the results of this study, we conclude that transplantation of VEGF165-transfected EPCs into the corpora cavernosa of rats with diabetic ED restores erectile function.
doi_str_mv 10.1038/aja.2010.116
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subjects Animals
Base Sequence
Diabetes Mellitus, Experimental - complications
Diabetes Mellitus, Experimental - physiopathology
DNA Primers - genetics
Endothelial Cells - physiology
Endothelial Cells - transplantation
Erectile Dysfunction - etiology
Erectile Dysfunction - physiopathology
Erectile Dysfunction - therapy
Male
Neovascularization, Physiologic
Original
Penile Erection - physiology
Penis - blood supply
Rats
Rats, Sprague-Dawley
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Stem Cell Transplantation
Transfection
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - physiology
内皮祖细胞
勃起功能障碍
基因转染
新生血管
糖尿病大鼠
细胞移植
血管内皮生长因子
阴茎海绵体
title Transplantation of endothelial progenitor cells transfected with VEGF165 to restore erectile function in diabetic rats
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