Neural progenitor cells labeling with microbubble contrast agent for ultrasound imaging in vivo

Abstract Tracking neuroprogenitor cells (NPCs) that are used to target tumors, infarction or inflammation, is paramount for cell-based therapy. We employed ultrasound imaging that can detect a single microbubble because it can distinguish its unique signal from those of surrounding tissues. NPCs eff...

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Bibliographic Details
Published in:Biomaterials 2013-07, Vol.34 (21), p.4926-4935
Main Authors: Cui, Wenjin, Tavri, Sidhartha, Benchimol, Michael J, Itani, Malak, Olson, Emilia S, Zhang, Hong, Decyk, Marika, Ramirez, Rosemarie G, Barback, Christopher V, Kono, Yuko, Mattrey, Robert F
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Biocompatibility
Biomedical materials
Bromodeoxyuridine - metabolism
Contrast agents
Contrast Media
Dentistry
Diagnostic Imaging - methods
DNA
Fluorescent Antibody Technique
Gene delivery
Imaging
In vivo testing
In vivo tests
Limit of Detection
Liver - pathology
Mice
Mice, Nude
Microbubble
Microbubbles
Neural progenitor cell
Neural Stem Cells - cytology
Neural Stem Cells - metabolism
Optical Phenomena
Staining and Labeling
Stem cell tracking
Surgical implants
Transfection
Ultrasonics - methods
Ultrasound
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Summary:Abstract Tracking neuroprogenitor cells (NPCs) that are used to target tumors, infarction or inflammation, is paramount for cell-based therapy. We employed ultrasound imaging that can detect a single microbubble because it can distinguish its unique signal from those of surrounding tissues. NPCs efficiently internalized positively charged microbubbles allowing a clinical ultrasound system to detect a single cell at 7 MHz. When injected intravenously, labeled NPCs traversed the lungs to be imaged in the left ventricle and the liver where they accumulated. Internalized microbubbles were not only less sensitive to destruction by ultrasound, but remained visible in vivo for days as compared to minutes when given free. The extended longevity provides ample time to allow cells to reach their intended target. We were also able to transfect NPCs in vitro when microbubbles were preloaded with GFP plasmid only when cells were insonated. Transfection efficiency and cell viability were both greater than 90%.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2013.03.020