Therapeutic Effect of the Tuber of Alisma orientale on Lipopolysaccharide-Induced Acute Lung Injury

Although Alisma orientale, an ethnic herb, has been prescribed for treating various diseases in Asian traditional medicine, experimental evidence to support its therapeutic effects is lacking. Here, we sought to determine whether A. orientale has a therapeutic effect on acute lung injury (ALI). Etha...

Full description

Saved in:
Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2013-01, Vol.2013 (2013), p.1-10
Main Authors: Kim, Kyun Ha, Kwun, Min Jung, Choi, Jun-Yong, Ahn, Kyung-Seop, Oh, Sei-Ryang, Lee, Yong Gyu, Christman, John W., Sadikot, Ruxana T., Han, Chang Woo, Joo, Myungsoo
Format: Article
Language:English
Subjects:
Alisma
Alveoli
Asthma
Bioluminescence
Bronchus
Chromatography
Cytokines
Disease
Drug dosages
Ethanol
High-performance liquid chromatography
Inflammation
Laboratory animals
Lethal dose
Leukocytes (neutrophilic)
Lipopolysaccharides
Liquid chromatography
Lungs
Medical research
Medicine
Mice
NF-κB protein
Polymerase chain reaction
Rodents
Sepsis
Spraying
TNF inhibitors
Trachea
Transgenic mice
Tumor necrosis factor-TNF
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Although Alisma orientale, an ethnic herb, has been prescribed for treating various diseases in Asian traditional medicine, experimental evidence to support its therapeutic effects is lacking. Here, we sought to determine whether A. orientale has a therapeutic effect on acute lung injury (ALI). Ethanol extract of the tuber of A. orientale (EEAO) was prepared and fingerprinted by HPLC for its constituents. Mice received an intraperitoneal (i.p.) injection of lipopolysaccharide (LPS) for the induction of ALI. At 2 h after LPS treatment, mice received an intratracheal (i.t.) spraying of various amounts of EEAO to the lung. Bioluminescence imaging of transgenic NF-κB/luciferase reporter mice shows that i.t. EEAO posttreatment suppressed lung inflammation. In similar experiments with C57BL/6 mice, EEAO posttreatment significantly improved lung inflammation, as assessed by H&E staining of lung sections, counting of neutrophils in bronchoalveolar lavage fluid, and semiquantitative RT-PCR analyses of proinflammatory cytokines and Nrf2-dependent genes in the inflamed lungs. Furthermore, EEAO posttreatment enhanced the survival of mice that received a lethal dose of LPS. Together, our results provide evidence that A. orientale has a therapeutic effect on ALI induced by sepsis.
ISSN:1741-427X
1741-4288
DOI:10.1155/2013/863892