Longitudinal transcriptomic dysregulation in the peripheral blood of transgenic Huntington's disease monkeys

• Published in: BMC neuroscience 2013-08, Vol.14 (1), p.88-88, Article 88
• Main Authors: Kocerha, Jannet, Liu, Yuhong, Willoughby, David, Chidamparam, Kumaravel, Benito, Joseph, Nelson, Kate, Xu, Yan, Chi, Tim, Engelhardt, Heidi, Moran, Sean, Yang, Shang-Hsun, Li, Shi-Hua, Li, Xiao-Jiang, Larkin, Katherine, Neumann, Adam, Banta, Heather, Yang, Jin Jing, Chan, Anthony W S
• Format: Article
• Language: English
• Subjects: Age; American Recovery & Reinvestment Act 2009-US; Animal cognition; Animal models; Animals; Animals, Genetically Modified; Blood; Candidates; Disease Models, Animal; Disease Progression; Epidemiology; Female; Gene expression; Gene Expression Profiling; Genes; Genetic aspects; Health aspects; High-definition television; Humans; Huntingtin Protein; Huntington Disease - blood; Huntington Disease - genetics; Huntington's chorea; Longitudinal Studies; Macaca mulatta; Male; Monkeys; Nerve Tissue Proteins - genetics; Physiological aspects; Primates; RNA; RNA, Messenger - metabolism; Studies; Transcriptome
• ISSN: 1471-2202; 1471-2202
• DOI: 10.1186/1471-2202-14-88

Huntington's Disease (HD) is a progressive neurodegenerative disorder caused by an expansion in the polyglutamine (polyQ) region of the Huntingtin (HTT) gene. The clinical features of HD are characterized by cognitive, psychological, and motor deficits. Molecular instability, a core component in neurological disease progression, can be comprehensively evaluated through longitudinal transcriptomic profiling. Development of animal models amenable to longitudinal examination enables distinct disease-associated mechanisms to be identified. Here we report the first longitudinal study of transgenic monkeys with genomic integration of various lengths of the human HTT gene and a range of polyQ repeats. With this unique group of transgenic HD nonhuman primates (HD monkeys), we profiled over 47,000 transcripts from peripheral blood collected over a 2 year timespan from HD monkeys and age-matched wild-type control monkeys. Messenger RNAs with expression patterns which diverged with disease progression in the HD monkeys considerably facilitated our search for transcripts with diagnostic or therapeutic potential in the blood of human HD patients, opening up a new avenue for clinical investigation.