Prolidase, matrix metalloproteinases 1 and 13 activity, oxidative-antioxidative status as a marker of preterm premature rupture of membranes and chorioamnionitis in maternal vaginal washing fluids

• Published in: International journal of medical sciences 2013-01, Vol.10 (10), p.1344-1351
• Main Authors: Soydinc, Hatice Ender, Sak, Muhammet Erdal, Evliyaoglu, Osman, Evsen, Mehmet Sıddık, Turgut, Abdulkadir, Özler, Ali, Yıldız, İsmail, Gul, Talip
• Format: Article
• Language: English
• Subjects: Antioxidants - metabolism; Aryldialkylphosphatase - metabolism; Female; Fetal Membranes, Premature Rupture - metabolism; Humans; Matrix Metalloproteinase 1 - metabolism; Matrix Metalloproteinase 13 - metabolism; Oxidative Stress - physiology; Pregnancy; Prospective Studies; Research Paper; Vagina - metabolism
• ISSN: 1449-1907; 1449-1907
• DOI: 10.7150/ijms.4802

Etiology of premature preterm rupture of membranes (PPROM) is not yet completely known and chorioamnionitis is one of the most important complications of its. We aimed to evaluate whether prolidase, matrix metalloproteinases, oxidative-antioxidative status, and inflammation markers in vaginal washing fluid (VWF) were associated with etiology of PPROM and whether these markers could be used to predict chorioamnionitis in PPROM. This prospective case control study enrolled fifty pregnant women with PPROM and 50 healthy pregnant women. The VWF samples were taken at the time of admission in the PPROM group and patients were followed for chorioamnionitis. Prolidase, matrix metalloproteinases, oxidative-antioxidative status, and inflammation markers in VWF were assayed. VWF levels of prolidase, matrix metalloproteinases 1-13 (p< 0.001), oxidative stress parameters, total oxidative stress (TOS) (p < 0.001) and oxidative stress index (OSI) (p = 0.002), and hs-CRP (p = 0.045) were significantly higher in the PPROM group than in the controls. Antioxidative status parameters, levels of paroxanase (PON-1) (p < 0.001) and total antioxidant capacity (TAC) (p < 0.001), were significantly lower in the PPROM group than in the controls. Mean VWF levels of prolidase (p < 0.001), metalloproteinases (p