Antigen-specific B memory cell responses to lipopolysaccharide (LPS) and invasion plasmid antigen (Ipa) B elicited in volunteers vaccinated with live-attenuated Shigella flexneri 2a vaccine candidates

Abstract We evaluated B memory responses in healthy adult volunteers who received one oral dose of live-attenuated Shigella flexneri 2a vaccine. LPS-specific BM cells increased from a median of 0 at baseline to 20 spot forming cells (SFC)/106 expanded cells following vaccination ( p = 0.008). A stro...

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Bibliographic Details
Published in:Vaccine 2009-01, Vol.27 (4), p.565-572
Main Authors: Simon, J.K, Wahid, R, Maciel, M, Picking, W.L, Kotloff, K.L, Levine, M.M, Sztein, M.B
Format: Article
Language:English
Subjects:
Administration, Oral
Adolescent
Adult
Allergy and Immunology
Antibodies
Antibodies, Bacterial - biosynthesis
Antibodies, Bacterial - blood
Antibodies, Bacterial - immunology
Antibody Specificity
Antigens
Antigens, Bacterial - genetics
Antigens, Bacterial - immunology
Applied microbiology
B cell memory
B-Lymphocytes - immunology
Bacterial Vaccines - administration & dosage
Bacterial Vaccines - immunology
Bacteriology
Biological and medical sciences
Dysentery, Bacillary - prevention & control
Female
Fundamental and applied biological sciences. Psychology
Health care
Humans
Immunization
Immunologic Memory
Laboratories
Lipopolysaccharides - immunology
Male
Microbiology
Middle Aged
Miscellaneous
Plasmids
Shigella
Shigella flexneri
Shigella flexneri - genetics
Shigella flexneri - immunology
Shigellosis
Vaccine
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Attenuated - administration & dosage
Vaccines, Attenuated - immunology
Volunteers
Young Adult
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Summary:Abstract We evaluated B memory responses in healthy adult volunteers who received one oral dose of live-attenuated Shigella flexneri 2a vaccine. LPS-specific BM cells increased from a median of 0 at baseline to 20 spot forming cells (SFC)/106 expanded cells following vaccination ( p = 0.008). A strong correlation was found between post-vaccination anti-LPS BM cell counts and peak serum anti-LPS IgG titers ( r s = 0.95, p = 0.0003). Increases in BM specific for IpaB approaching significance were also observed. In sum, oral vaccination with live-attenuated S. flexneri 2a elicits BM cells to LPS and IpaB, suggesting that BM responses to Shigella antigens should be further studied as a suitable surrogate of protection in shigellosis.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2008.10.081