Functional Interactions of VirB11 Traffic ATPases with VirB4 and VirD4 Molecular Motors in Type IV Secretion Systems

Pilus biogenesis and substrate transport by type IV secretion systems require energy, which is provided by three molecular motors localized at the base of the secretion channel. One of these motors, VirB11, belongs to the superfamily of traffic ATPases, which includes members of the type II secretio...

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Bibliographic Details
Published in:Journal of Bacteriology 2013-09, Vol.195 (18), p.4195-4201
Main Authors: Ripoll-Rozada, Jorge, Zunzunegui, Sandra, de la Cruz, Fernando, Arechaga, Ignacio, Cabezón, Elena
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
Adenosine Triphosphatases - chemistry
Adenosine Triphosphatases - genetics
Adenosine Triphosphatases - metabolism
adenosinetriphosphatase
Agrobacterium tumefaciens - genetics
Agrobacterium tumefaciens - metabolism
Amino Acid Sequence
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacterial Secretion Systems - genetics
Bacteriology
biogenesis
Biological Transport
Biosynthesis
Conjugation, Genetic
Deoxyribonucleic acid
DNA
energy
fimbriae
Fimbriae, Bacterial - genetics
Fimbriae, Bacterial - metabolism
kinesin
Models, Molecular
Molecular Motor Proteins - chemistry
Molecular Motor Proteins - genetics
Molecular Motor Proteins - metabolism
Mutation
Phylogenetics
phylogeny
Plasmids
Protein Interaction Domains and Motifs
Proteins
secretion
transfer DNA
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Summary:Pilus biogenesis and substrate transport by type IV secretion systems require energy, which is provided by three molecular motors localized at the base of the secretion channel. One of these motors, VirB11, belongs to the superfamily of traffic ATPases, which includes members of the type II secretion system and the type IV pilus and archaeal flagellar assembly apparatus. Here, we report the functional interactions between TrwD, the VirB11 homolog of the conjugative plasmid R388, and TrwK and TrwB, the motors involved in pilus biogenesis and DNA transport, respectively. Although these interactions remained standing upon replacement of the traffic ATPase by a homolog from a phylogenetically related conjugative system, namely, TraG of plasmid pKM101, this homolog could not replace the TrwD function for DNA transfer. This result suggests that VirB11 works as a switch between pilus biogenesis and DNA transport and reinforces a mechanistic model in which VirB11 proteins act as traffic ATPases by regulating both events in type IV secretion systems.
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/JB.00437-13