Expression of klotho mRNA and protein in rat brain parenchyma from early postnatal development into adulthood

Abstract Without the age-regulating protein klotho, mouse lifespan is shortened and the rapid onset of age-related disorders occurs. Conversely, overexpression of klotho extends mouse lifespan. Klotho is most abundant in kidney and expressed in a limited number of other organs, including the brain,...

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Bibliographic Details
Published in:Brain research 2013-08, Vol.1527, p.1-14
Main Authors: Clinton, Sarah M, Glover, Matthew E, Maltare, Astha, Laszczyk, Ann M, Mehi, Stephen J, Simmons, Rebecca K, King, Gwendalyn D
Format: Article
Language:English
Subjects:
adulthood
adults
Aging
Aging - physiology
amygdala
Animals
Biological and medical sciences
Brain - growth & development
Brain - metabolism
choroid plexus
cortex
Development. Senescence. Regeneration. Transplantation
Fundamental and applied biological sciences. Psychology
gene expression
Glucuronidase - analysis
Glucuronidase - biosynthesis
hippocampus
Immunohistochemistry
In Situ Hybridization
kidneys
longevity
Male
mice
Neurodevelopment
Neurology
parenchyma
postnatal development
protein synthesis
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Vertebrates: nervous system and sense organs
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Summary:Abstract Without the age-regulating protein klotho, mouse lifespan is shortened and the rapid onset of age-related disorders occurs. Conversely, overexpression of klotho extends mouse lifespan. Klotho is most abundant in kidney and expressed in a limited number of other organs, including the brain, where klotho levels are highest in choroid plexus. Reports vary on where klotho is expressed within the brain parenchyma, and no data is available as to whether klotho levels change across postnatal development. We used in situ hybridization to map klotho mRNA expression in the developing and adult rat brain and report moderate, widespread expression across grey matter regions. mRNA expression levels in cortex, hippocampus, caudate putamen, and amygdala decreased during the second week of life and then gradually rose to adult levels by postnatal day 21. Immunohistochemistry revealed a protein expression pattern similar to the mRNA results, with klotho protein expressed widely throughout the brain. Klotho protein co-localized with both the neuronal marker NeuN, as well as, oligodendrocyte marker olig2. These results provide the first anatomical localization of klotho mRNA and protein in rat brain parenchyma and demonstrate that klotho levels vary during early postnatal development.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2013.06.044