Near-infrared imaging of face transplants: are both pedicles necessary?

• Published in: The Journal of surgical research 2013-09, Vol.184 (1), p.714-721
• Main Authors: Nguyen, John T., MD, Ashitate, Yoshitomo, MD, Venugopal, Vivek, PhD, Neacsu, Florin, MS, Kettenring, Frank, BS, Frangioni, John V., MD, PhD, Gioux, Sylvain, PhD, Lee, Bernard T., MD, MBA
• Format: Article
• Language: English
• Subjects: Animals; Composite tissue allotransplantation; Face - blood supply; Face - surgery; Face transplantation; Facial Transplantation - methods; Female; Fluorescence; Microsurgery - methods; Models, Animal; Near infrared imaging; Neck - blood supply; Spectroscopy, Near-Infrared - methods; Surgery; Surgical Flaps - blood supply; Sus scrofa; Tissue and Organ Harvesting - methods; Transplantation, Homologous
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2013.04.039

Abstract Background Facial transplantation is a complex procedure that corrects severe facial defects due to traumas, burns, and congenital disorders. Although face transplantation has been successfully performed clinically, potential risks include tissue ischemia and necrosis. The vascular supply is typically based on the bilateral neck vessels. As it remains unclear whether perfusion can be based off a single pedicle, this study was designed to assess perfusion patterns of facial transplant allografts using near-infrared (NIR) fluorescence imaging. Methods Upper facial composite tissue allotransplants were created using both carotid artery and external jugular vein pedicles in Yorkshire pigs. A flap validation model was created in n = 2 pigs and a clamp occlusion model was performed in n = 3 pigs. In the clamp occlusion models, sequential clamping of the vessels was performed to assess perfusion. Animals were injected with indocyanine green and imaged with NIR fluorescence. Quantitative metrics were assessed based on fluorescence intensity. Results With NIR imaging, arterial perforators emitted fluorescence indicating perfusion along the surface of the skin. Isolated clamping of one vascular pedicle showed successful perfusion across the midline based on NIR fluorescence imaging. This perfusion extended into the facial allograft within 60 s and perfused the entire contralateral side within 5 min. Conclusions Determination of vascular perfusion is important in microsurgical constructs as complications can lead to flap loss. It is still unclear if facial transplants require both pedicles. This initial pilot study using intraoperative NIR fluorescence imaging suggests that facial flap models can be adequately perfused from a single pedicle.