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Berberine Reduces the Metastasis of Chondrosarcoma by Modulating the αvβ3 Integrin and the PKCδ, c-Src, and AP-1 Signaling Pathways
Chondrosarcoma is a primary malignant bone cancer, with a potent capacity to invade locally and cause distant metastasis, especially to the lungs. Patients diagnosed with chondrosarcoma have poor prognosis. Berberine, an active component of the Ranunculaceae and Papaveraceae families of plant, has b...
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Published in: | Evidence-based complementary and alternative medicine 2013-01, Vol.2013 (2013), p.1-10 |
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description | Chondrosarcoma is a primary malignant bone cancer, with a potent capacity to invade locally and cause distant metastasis, especially to the lungs. Patients diagnosed with chondrosarcoma have poor prognosis. Berberine, an active component of the Ranunculaceae and Papaveraceae families of plant, has been proven to induce tumor apoptosis and to prevent the metastasis of cancer cells. However, the effects of berberine in human chondrosarcoma are largely unknown. In this study, we found that berberine did not induce cell apoptosis in human primary chondrocytes and chondrosarcoma cells. However, at noncytotoxic concentrations, berberine reduced the migration and invasion of chondrosarcoma cancer cells. Integrins are the major adhesive molecules in mammalian cells and have been associated with the metastasis of cancer cells. We also found that incubation of chondrosarcoma cells with berberine reduced mRNA transcription for, and cell surface expression of, the αvβ3 integrin, with additional inhibitory effects on PKCδ, c-Src, and NF-κB activation. Thus, berberine may be a novel antimetastasis agent for the treatment of metastatic chondrosarcoma. |
doi_str_mv | 10.1155/2013/423164 |
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Patients diagnosed with chondrosarcoma have poor prognosis. Berberine, an active component of the Ranunculaceae and Papaveraceae families of plant, has been proven to induce tumor apoptosis and to prevent the metastasis of cancer cells. However, the effects of berberine in human chondrosarcoma are largely unknown. In this study, we found that berberine did not induce cell apoptosis in human primary chondrocytes and chondrosarcoma cells. However, at noncytotoxic concentrations, berberine reduced the migration and invasion of chondrosarcoma cancer cells. Integrins are the major adhesive molecules in mammalian cells and have been associated with the metastasis of cancer cells. We also found that incubation of chondrosarcoma cells with berberine reduced mRNA transcription for, and cell surface expression of, the αvβ3 integrin, with additional inhibitory effects on PKCδ, c-Src, and NF-κB activation. Thus, berberine may be a novel antimetastasis agent for the treatment of metastatic chondrosarcoma.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2013/423164</identifier><identifier>PMID: 24027594</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Activator protein 1 ; Apoptosis ; Berberine ; Bone cancer ; Bone tumors ; Cancer therapies ; Cell adhesion ; Cell adhesion & migration ; Cell culture ; Cell cycle ; Cell surface ; Chondrocytes ; Chondrosarcoma ; Enzymes ; Integrins ; Kinases ; Mammalian cells ; Medical research ; Metastases ; Metastasis ; Motility ; NF-κB protein ; Prostate cancer ; Protein kinase C ; Proteins ; Signal transduction ; Src protein ; Transcription factors</subject><ispartof>Evidence-based complementary and alternative medicine, 2013-01, Vol.2013 (2013), p.1-10</ispartof><rights>Copyright © 2013 Chi-Ming Wu et al.</rights><rights>Copyright © 2013 Chi-Ming Wu et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2013 Chi-Ming Wu et al. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-8326-1489</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1710735505/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1710735505?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,25753,27924,27925,37012,44590,74998</link.rule.ids></links><search><contributor>Yang, Shun-Fa</contributor><contributor>Shun-Fa Yang</contributor><creatorcontrib>Wu, Chi-Ming</creatorcontrib><creatorcontrib>Li, Te-Mao</creatorcontrib><creatorcontrib>Tan, Tzu-Wei</creatorcontrib><creatorcontrib>Fong, Yi-Chin</creatorcontrib><creatorcontrib>Tang, Chih-Hsin</creatorcontrib><title>Berberine Reduces the Metastasis of Chondrosarcoma by Modulating the αvβ3 Integrin and the PKCδ, c-Src, and AP-1 Signaling Pathways</title><title>Evidence-based complementary and alternative medicine</title><description>Chondrosarcoma is a primary malignant bone cancer, with a potent capacity to invade locally and cause distant metastasis, especially to the lungs. Patients diagnosed with chondrosarcoma have poor prognosis. Berberine, an active component of the Ranunculaceae and Papaveraceae families of plant, has been proven to induce tumor apoptosis and to prevent the metastasis of cancer cells. However, the effects of berberine in human chondrosarcoma are largely unknown. In this study, we found that berberine did not induce cell apoptosis in human primary chondrocytes and chondrosarcoma cells. However, at noncytotoxic concentrations, berberine reduced the migration and invasion of chondrosarcoma cancer cells. Integrins are the major adhesive molecules in mammalian cells and have been associated with the metastasis of cancer cells. We also found that incubation of chondrosarcoma cells with berberine reduced mRNA transcription for, and cell surface expression of, the αvβ3 integrin, with additional inhibitory effects on PKCδ, c-Src, and NF-κB activation. Thus, berberine may be a novel antimetastasis agent for the treatment of metastatic chondrosarcoma.</description><subject>Activator protein 1</subject><subject>Apoptosis</subject><subject>Berberine</subject><subject>Bone cancer</subject><subject>Bone tumors</subject><subject>Cancer therapies</subject><subject>Cell adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell surface</subject><subject>Chondrocytes</subject><subject>Chondrosarcoma</subject><subject>Enzymes</subject><subject>Integrins</subject><subject>Kinases</subject><subject>Mammalian cells</subject><subject>Medical research</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Motility</subject><subject>NF-κB protein</subject><subject>Prostate cancer</subject><subject>Protein kinase C</subject><subject>Proteins</subject><subject>Signal transduction</subject><subject>Src protein</subject><subject>Transcription factors</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNpVkdtKHEEQhhtRotFc5Vpo8C442j19mrkRzBKjRHGJEXLX9PRhp2W3W7tnlH0B3ychz7HP5OyuLAQKqqj66i-KH4DPGJ1gzNhpiTA5pSXBnG6BPSwoLmhZVdubWvzeBR9zfkCorIUQH8BuSVEpWE33wOtXmxqbfLDwpzW9thl2rYU3tlN5CJ9hdHDUxmBSzCrpOFOwmcObaPqp6nyYrPDFn-fFXwKvQmcngxZUwaz64x-jxb9jqIu7pI9X3fNxgeGdnwQ1XS6PVde-qHk-ADtOTbP99J73wf3Ft1-jy-L69vvV6Py6sFgIWlDOhcAOO6ItZbq2vOHMsdpxVLoKKa4pMzWrccUcEsYJSogpK9EwzpTmhuyDs7XuY9_MrNE2dElN5WPyM5XmMiov_58E38pJfJZEcMJ4PQgcvQuk-NTb3MmH2KfhmyyxwEgQxhAbqC9rqvXBqBe_OYCRXHoml57JtWcDfLiG7YBYpzYwFaiuOHkDeOKS-A</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Wu, Chi-Ming</creator><creator>Li, Te-Mao</creator><creator>Tan, Tzu-Wei</creator><creator>Fong, Yi-Chin</creator><creator>Tang, Chih-Hsin</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8326-1489</orcidid></search><sort><creationdate>20130101</creationdate><title>Berberine Reduces the Metastasis of Chondrosarcoma by Modulating the αvβ3 Integrin and the PKCδ, c-Src, and AP-1 Signaling Pathways</title><author>Wu, Chi-Ming ; Li, Te-Mao ; Tan, Tzu-Wei ; Fong, Yi-Chin ; Tang, Chih-Hsin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e1774-466771f1f3ce45c9e6b65f59f602f80a6c45d959185f07df7433d287b565ac6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Activator protein 1</topic><topic>Apoptosis</topic><topic>Berberine</topic><topic>Bone cancer</topic><topic>Bone tumors</topic><topic>Cancer therapies</topic><topic>Cell adhesion</topic><topic>Cell adhesion & migration</topic><topic>Cell culture</topic><topic>Cell cycle</topic><topic>Cell surface</topic><topic>Chondrocytes</topic><topic>Chondrosarcoma</topic><topic>Enzymes</topic><topic>Integrins</topic><topic>Kinases</topic><topic>Mammalian cells</topic><topic>Medical research</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Motility</topic><topic>NF-κB protein</topic><topic>Prostate cancer</topic><topic>Protein kinase C</topic><topic>Proteins</topic><topic>Signal transduction</topic><topic>Src protein</topic><topic>Transcription factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Chi-Ming</creatorcontrib><creatorcontrib>Li, Te-Mao</creatorcontrib><creatorcontrib>Tan, Tzu-Wei</creatorcontrib><creatorcontrib>Fong, Yi-Chin</creatorcontrib><creatorcontrib>Tang, Chih-Hsin</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Psychology Database (ProQuest)</collection><collection>ProQuest Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Chi-Ming</au><au>Li, Te-Mao</au><au>Tan, Tzu-Wei</au><au>Fong, Yi-Chin</au><au>Tang, Chih-Hsin</au><au>Yang, Shun-Fa</au><au>Shun-Fa Yang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Berberine Reduces the Metastasis of Chondrosarcoma by Modulating the αvβ3 Integrin and the PKCδ, c-Src, and AP-1 Signaling Pathways</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><date>2013-01-01</date><risdate>2013</risdate><volume>2013</volume><issue>2013</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Chondrosarcoma is a primary malignant bone cancer, with a potent capacity to invade locally and cause distant metastasis, especially to the lungs. Patients diagnosed with chondrosarcoma have poor prognosis. Berberine, an active component of the Ranunculaceae and Papaveraceae families of plant, has been proven to induce tumor apoptosis and to prevent the metastasis of cancer cells. However, the effects of berberine in human chondrosarcoma are largely unknown. In this study, we found that berberine did not induce cell apoptosis in human primary chondrocytes and chondrosarcoma cells. However, at noncytotoxic concentrations, berberine reduced the migration and invasion of chondrosarcoma cancer cells. Integrins are the major adhesive molecules in mammalian cells and have been associated with the metastasis of cancer cells. We also found that incubation of chondrosarcoma cells with berberine reduced mRNA transcription for, and cell surface expression of, the αvβ3 integrin, with additional inhibitory effects on PKCδ, c-Src, and NF-κB activation. Thus, berberine may be a novel antimetastasis agent for the treatment of metastatic chondrosarcoma.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>24027594</pmid><doi>10.1155/2013/423164</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-8326-1489</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Activator protein 1 Apoptosis Berberine Bone cancer Bone tumors Cancer therapies Cell adhesion Cell adhesion & migration Cell culture Cell cycle Cell surface Chondrocytes Chondrosarcoma Enzymes Integrins Kinases Mammalian cells Medical research Metastases Metastasis Motility NF-κB protein Prostate cancer Protein kinase C Proteins Signal transduction Src protein Transcription factors |
title | Berberine Reduces the Metastasis of Chondrosarcoma by Modulating the αvβ3 Integrin and the PKCδ, c-Src, and AP-1 Signaling Pathways |
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