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Berberine Reduces the Metastasis of Chondrosarcoma by Modulating the αvβ3 Integrin and the PKCδ, c-Src, and AP-1 Signaling Pathways

Chondrosarcoma is a primary malignant bone cancer, with a potent capacity to invade locally and cause distant metastasis, especially to the lungs. Patients diagnosed with chondrosarcoma have poor prognosis. Berberine, an active component of the Ranunculaceae and Papaveraceae families of plant, has b...

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Published in:Evidence-based complementary and alternative medicine 2013-01, Vol.2013 (2013), p.1-10
Main Authors: Wu, Chi-Ming, Li, Te-Mao, Tan, Tzu-Wei, Fong, Yi-Chin, Tang, Chih-Hsin
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Li, Te-Mao
Tan, Tzu-Wei
Fong, Yi-Chin
Tang, Chih-Hsin
description Chondrosarcoma is a primary malignant bone cancer, with a potent capacity to invade locally and cause distant metastasis, especially to the lungs. Patients diagnosed with chondrosarcoma have poor prognosis. Berberine, an active component of the Ranunculaceae and Papaveraceae families of plant, has been proven to induce tumor apoptosis and to prevent the metastasis of cancer cells. However, the effects of berberine in human chondrosarcoma are largely unknown. In this study, we found that berberine did not induce cell apoptosis in human primary chondrocytes and chondrosarcoma cells. However, at noncytotoxic concentrations, berberine reduced the migration and invasion of chondrosarcoma cancer cells. Integrins are the major adhesive molecules in mammalian cells and have been associated with the metastasis of cancer cells. We also found that incubation of chondrosarcoma cells with berberine reduced mRNA transcription for, and cell surface expression of, the αvβ3 integrin, with additional inhibitory effects on PKCδ, c-Src, and NF-κB activation. Thus, berberine may be a novel antimetastasis agent for the treatment of metastatic chondrosarcoma.
doi_str_mv 10.1155/2013/423164
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Patients diagnosed with chondrosarcoma have poor prognosis. Berberine, an active component of the Ranunculaceae and Papaveraceae families of plant, has been proven to induce tumor apoptosis and to prevent the metastasis of cancer cells. However, the effects of berberine in human chondrosarcoma are largely unknown. In this study, we found that berberine did not induce cell apoptosis in human primary chondrocytes and chondrosarcoma cells. However, at noncytotoxic concentrations, berberine reduced the migration and invasion of chondrosarcoma cancer cells. Integrins are the major adhesive molecules in mammalian cells and have been associated with the metastasis of cancer cells. We also found that incubation of chondrosarcoma cells with berberine reduced mRNA transcription for, and cell surface expression of, the αvβ3 integrin, with additional inhibitory effects on PKCδ, c-Src, and NF-κB activation. Thus, berberine may be a novel antimetastasis agent for the treatment of metastatic chondrosarcoma.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2013/423164</identifier><identifier>PMID: 24027594</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Activator protein 1 ; Apoptosis ; Berberine ; Bone cancer ; Bone tumors ; Cancer therapies ; Cell adhesion ; Cell adhesion &amp; migration ; Cell culture ; Cell cycle ; Cell surface ; Chondrocytes ; Chondrosarcoma ; Enzymes ; Integrins ; Kinases ; Mammalian cells ; Medical research ; Metastases ; Metastasis ; Motility ; NF-κB protein ; Prostate cancer ; Protein kinase C ; Proteins ; Signal transduction ; Src protein ; Transcription factors</subject><ispartof>Evidence-based complementary and alternative medicine, 2013-01, Vol.2013 (2013), p.1-10</ispartof><rights>Copyright © 2013 Chi-Ming Wu et al.</rights><rights>Copyright © 2013 Chi-Ming Wu et al. 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subjects Activator protein 1
Apoptosis
Berberine
Bone cancer
Bone tumors
Cancer therapies
Cell adhesion
Cell adhesion & migration
Cell culture
Cell cycle
Cell surface
Chondrocytes
Chondrosarcoma
Enzymes
Integrins
Kinases
Mammalian cells
Medical research
Metastases
Metastasis
Motility
NF-κB protein
Prostate cancer
Protein kinase C
Proteins
Signal transduction
Src protein
Transcription factors
title Berberine Reduces the Metastasis of Chondrosarcoma by Modulating the αvβ3 Integrin and the PKCδ, c-Src, and AP-1 Signaling Pathways
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