Surface plasmon resonance imaging of cell-substrate contacts with radially polarized beams

We demonstrate the proof-of-concept for surface plasmon resonance sensing and imaging via a virtual probe at the cell-substrate interface of a biological cell in aqueous media. The technique is based on the optical excitation by focused radially polarized beams of localized surface plasmons, which f...

Full description

Saved in:
Bibliographic Details
Published in:Optics express 2008-12, Vol.16 (25), p.20734-20741
Main Authors: Moh, K J, Yuan, X-C, Bu, J, Zhu, S W, Gao, Bruce Z
Format: Article
Language:English
Subjects:
Cell Membrane - ultrastructure
Image Interpretation, Computer-Assisted - methods
Microscopy, Confocal - methods
Refractometry - methods
Surface Plasmon Resonance - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We demonstrate the proof-of-concept for surface plasmon resonance sensing and imaging via a virtual probe at the cell-substrate interface of a biological cell in aqueous media. The technique is based on the optical excitation by focused radially polarized beams of localized surface plasmons, which forms a virtual probe on the metal substrate. The intensity distribution at the back focal plane of the objective lens enables quantitative measurements to be made of the cell-substrate contact. The acquired data is then visualized in the form of a local refractive index map.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.16.020734