Development of Metabolic Function Biomarkers in the Common Marmoset, Callithrix jacchus

Metabolic assessment of a non‐human primate model of metabolic syndrome and obesity requires the necessary biomarkers specific to the species. While the rhesus monkey has a number of specific assays for assessing metabolic syndrome, the marmoset does not. Furthermore, the common marmoset (Callithrix...

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Bibliographic Details
Published in:American journal of primatology 2013-05, Vol.75 (5), p.500-508
Main Authors: ZIEGLER, TONI E., COLMAN, RICKI J., TARDIF, SUZETTE D., SOSA, MEGAN E., WEGNER, FREDRICK H., WITTWER, DANIEL J., SHRESTHA, HEMANTA
Format: Article
Language:English
Subjects:
adiponectin
Adiponectin - blood
Animals
Biomarkers
Blood
Callithrix - blood
Callithrix - physiology
Diseases
Energy Metabolism - physiology
Female
ghrelin
Ghrelin - blood
Immunoassay - methods
Immunoassay - veterinary
insulin
Insulin - blood
leptin
Leptin - blood
Male
marmoset
metabolic syndrome
New World monkeys
Obesity
Primate behaviour
Primate biology
Reproducibility of Results
Sex Factors
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Summary:Metabolic assessment of a non‐human primate model of metabolic syndrome and obesity requires the necessary biomarkers specific to the species. While the rhesus monkey has a number of specific assays for assessing metabolic syndrome, the marmoset does not. Furthermore, the common marmoset (Callithrix jacchus) has a small blood volume that necessitates using a single blood volume for multiple analyses. The common marmoset holds a great potential as an alternative primate model for the study of human disease but assay methods need to be developed and validated for the biomarkers of metabolic syndrome. Here we report on the adaptation, development, and validation of commercially available immunoassays for common marmoset samples in small volumes. We have performed biological validations for insulin, adiponectin, leptin, and ghrelin to demonstrate the use of these biomarkers in examining metabolic syndrome and other related diseases in the common marmoset. Am. J. Primatol. 75:500‐508, 2013. © 2013 Wiley Periodicals, Inc.
ISSN:0275-2565
1098-2345
DOI:10.1002/ajp.22126