Evaluation of the Pan Influenza detection kit utilizing the PLEX-ID and influenza samples from the 2011 respiratory season

•Overall agreement was 89.3% (67/75 kappa=0.57 95% CI 0.3–0.89).•Positive percent agreement was 92.3% (60/65).•H1N1 pdm09 was identified in 42.6% (32/75) of samples tested.•A/NEW YORK/15/2009(H1N1 pdm09) was the most prevalent H1N1 genotype.•A/Thailand/CU-B1697/2009 was the most prevalent H3N2 genot...

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Bibliographic Details
Published in:Journal of virological methods 2013-10, Vol.193 (1), p.173-176
Main Authors: A. Murillo, Luis, Hardick, Justin, Jeng, Kevin, Gaydos, Charlotte A.
Format: Article
Language:English
Subjects:
genes
Genotype
Genotyping
H1N1 pdm09
Humans
influenza
Influenza A virus
Influenza A virus - genetics
Influenza A virus - isolation & purification
Influenza, Human - diagnosis
Influenza, Human - virology
Molecular Diagnostic Techniques - methods
Nasopharynx - virology
Orthomyxoviridae
PLEX-ID
reverse transcriptase polymerase chain reaction
Virology - methods
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Summary:•Overall agreement was 89.3% (67/75 kappa=0.57 95% CI 0.3–0.89).•Positive percent agreement was 92.3% (60/65).•H1N1 pdm09 was identified in 42.6% (32/75) of samples tested.•A/NEW YORK/15/2009(H1N1 pdm09) was the most prevalent H1N1 genotype.•A/Thailand/CU-B1697/2009 was the most prevalent H3N2 genotype. A comparison study was performed between the PLEX-ID and the CDC RT-PCR method for the detection and identification of Influenza A viruses using nasopharyngeal samples (N=75) collected between January and May 2011. Overall agreement was 89.3% (67/75 kappa=0.57 95% CI 0.3–0.89). Positive percent agreement was 92.3% (60/65); negative percent agreement was 70% (7/10). H1N1 pdm09 identified: 42.6% (32/75) and 54.7% (41/75) by PLEX-ID and CDC RT-PCR, respectively. H3N2 identified: 29.3% (22/75) and 32% (24/75) of samples by PLEX-ID and CDC RT-PCR, respectively. Negatives identified: 16% (12/75) and 13.3% (10/75), by PLEX-ID and CDC RT-PCR respectively. For influenza viruses identified as H1N1 pdm09, Influenza A virus A/NEW YORK/15/2009(H1N1 pdm09) was the most prevalent genotype at 50% (16/32), followed by A/CALIFORNIA/05/2009(H1N1 pdm09) at 18.2% (6/32). Updated assay plates containing additional primers designed for H1N1 pdm09 HA and NA genes were utilized for this evaluation. Among H1N1 pdm09 samples, the HA gene was conserved in 96.9% (31/32) of samples. The NA gene was conserved in 96.9% (31/32).
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2013.06.006