Induction of apoptosis and the regulation of ErbB signaling by laminarin in HT-29 human colon cancer cells

Laminarin, found in marine brown algae, is used as a carbohydrate reserve for phytoplankton; however, it is also used in traditional Chinese medicine, and has been shown to have several biological activities, including anticancer activities. In this study, we examined the mechanisms through which la...

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Bibliographic Details
Published in:International journal of molecular medicine 2013-08, Vol.32 (2), p.291-295
Main Authors: PARK, HEE-KYOUNG, KIM, IN-HYE, KIM, JOONGKYUN, NAM, TAEK-JEONG
Format: Article
Language:English
Subjects:
Algae
Apoptosis
Cell cycle
Cell growth
Colorectal cancer
Cytochrome
Epidermal growth factor
ErbB signaling pathway
Immunoglobulins
Insulin-like growth factors
Kinases
laminarin
Ligands
Membranes
Phosphorylation
Protein expression
Proteins
Signal transduction
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Summary:Laminarin, found in marine brown algae, is used as a carbohydrate reserve for phytoplankton; however, it is also used in traditional Chinese medicine, and has been shown to have several biological activities, including anticancer activities. In this study, we examined the mechanisms through which laminarin from Laminaria digitata induces apoptosis in HT-29 colon cancer cells, as well as the involvement of the ErbB signaling pathway. Cell viability assay revealed that laminarin induced cell death in a dose-dependent manner. Cell cycle analysis revealed that laminarin increased the percentage of cells in the sub-G1 and G2-M phase. Western blot analysis demonstrated that laminarin inhibited the heregulin-stimulated phosphorylation of ErbB2. A decrease in cellular proliferation was also observed; this was found to be dependent on ErbB, which activates c-Jun N-terminal kinase. These findings demonstrate the important role of the epidermal growth factor receptor in colon cancer tumorigenesis, and suggest the potential of laminarin as a bio-functional food with anticancer effects on human colon cancer.
ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.2013.1409