Cochaperonin CPN20 negatively regulates abscisic acid signaling in Arabidopsis

Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction protein, the chloroplast co-chaperonin 20 (CPN20) in ABA signaling in Arabidopsis thaliana . W...

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Bibliographic Details
Published in:Plant molecular biology 2013-10, Vol.83 (3), p.205-218
Main Authors: Zhang, Xiao-Feng, Jiang, Tao, Wu, Zhen, Du, Shu-Yuan, Yu, Yong-Tao, Jiang, Shang-Chuan, Lu, Kai, Feng, Xiu-Jing, Wang, Xiao-Fang, Zhang, Da-Peng
Format: Article
Language:English
Subjects:
abscisic acid
Abscisic Acid - metabolism
Arabidopsis
Arabidopsis - metabolism
Arabidopsis Proteins
Arabidopsis Proteins - genetics
Arabidopsis Proteins - physiology
Arabidopsis thaliana
Biochemistry
Biomedical and Life Sciences
Down-Regulation
gene overexpression
genes
genetics
Group I Chaperonins
Group I Chaperonins - genetics
Group I Chaperonins - physiology
Life Sciences
Lyases
Lyases - metabolism
Magnesium
metabolism
physiology
Phytochemicals
Plant biology
Plant Pathology
Plant reproduction
plant response
Plant Sciences
Seed germination
Signal Transduction
stomatal movement
transcription (genetics)
transcription factors
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Summary:Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction protein, the chloroplast co-chaperonin 20 (CPN20) in ABA signaling in Arabidopsis thaliana . We showed that down-expression of the CPN20 gene increases, but overexpression of the CPN20 gene reduces, ABA sensitivity in the major ABA responses including ABA-induced seed germination inhibition, postgermination growth arrest, promotion of stomatal closure and inhibition of stomatal opening. Genetic evidence supports that CPN20 functions downstream or at the same node of CHLH/ABAR, but upstream of the WRKY40 transcription factor. The other CPN20 interaction partners CPN10 and CPN60 are not involved in ABA signaling. Our findings show that CPN20 functions negatively in the ABAR-WRKY40 coupled ABA signaling independently of its co-chaperonin role, and provide a new insight into the role of co-chaperones in the regulation of plant responses to environmental cues.
ISSN:0167-4412
1573-5028
DOI:10.1007/s11103-013-0082-8