Aggravating Genetic Interactions Allow a Solution to Redundancy in a Bacterial Pathogen

Interactions between hosts and pathogens are complex, so understanding the events that govern these interactions requires the analysis of molecular mechanisms operating in both organisms. Many pathogens use multiple strategies to target a single event in the disease process, confounding the identifi...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2012-12, Vol.338 (6113), p.1440-1444
Main Authors: O'Connor, Tamara J., Boyd, Dana, Dorer, Marion S., Isberg, Ralph R.
Format: Article
Language:English
Subjects:
Animals
Bacteria
Bacterial Proteins - genetics
Bacterial Secretion Systems - genetics
Bacteriology
Biological and medical sciences
Cell growth
Cells, Cultured
Chlamydia
Drosophila
Drosophila melanogaster - cytology
Flavoproteins - genetics
Functional groups
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic mutation
Genetic Testing - methods
Genetics
Host-Pathogen Interactions - genetics
Humans
Legionella
Legionella pneumophila
Legionella pneumophila - genetics
Legionella pneumophila - growth & development
Macrophages
Macrophages - microbiology
Microbial genetics
Microbiology
Multivariate Analysis
Mutagenesis
Mutagenesis, Insertional - methods
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Pathogens
RESEARCH ARTICLE
RNA Interference
Salmonella
Sequence Deletion
Vacuoles
Vacuoles - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Interactions between hosts and pathogens are complex, so understanding the events that govern these interactions requires the analysis of molecular mechanisms operating in both organisms. Many pathogens use multiple strategies to target a single event in the disease process, confounding the identification of the important determinants of virulence. We developed a genetic screening strategy called insertional mutagenesis and depletion (iMAD) that combines bacterial mutagenesis and RNA interference, to systematically dissect the interplay between a pathogen and its host. We used this technique to resolve the network of proteins secreted by the bacterium Legionella pneumophila to promote intracellular growth, a critical determinant of pathogenicity of this organism. This strategy is broadly applicable, allowing the dissection of any interface between two organisms involving numerous interactions.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1229556