Highly Efficient Bienzyme Functionalized Nanocomposite-Based Microfluidics Biosensor Platform for Biomedical Application

• Published in: Scientific reports 2013-09, Vol.3 (1), p.2661, Article 2661
• Main Authors: Ali, Md. Azahar, Srivastava, Saurabh, Solanki, Pratima R., Reddy, Venu, Agrawal, Ved V., Kim, CheolGi, John, Renu, Malhotra, Bansi D.
• Format: Article
• Language: English
• Subjects: 639/638/161/886; 639/925/350/877; 639/925/927/356; 639/925/927/59; Biosensing Techniques - instrumentation; Biosensors; Cholesterol; Cholesterol oxidase; Cholesterol Oxidase - chemistry; Cholesterol Oxidase - metabolism; Electrodes; Electron microscopy; Enzymes - chemistry; Enzymes - metabolism; Esterase; Fabrication; Humanities and Social Sciences; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Microfluidics; Microfluidics - instrumentation; multidisciplinary; Nanocomposites; Nanocomposites - chemistry; Nanocomposites - ultrastructure; Nanoparticles; Nanotechnology; Nanotubes; Nanotubes, Carbon - chemistry; Nanotubes, Carbon - ultrastructure; Nickel; Nickel - chemistry; Photoelectron Spectroscopy; Polydimethylsiloxane; Scanning electron microscopy; Science; Spectroscopy; Spectrum Analysis, Raman; Sterol Esterase - chemistry; Sterol Esterase - metabolism; X-Ray Diffraction; X-rays
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep02661

This report describes the fabrication of a novel microfluidics nanobiochip based on a composite comprising of nickel oxide nanoparticles (nNiO) and multiwalled carbon nanotubes (MWCNTs), as well as the chip's use in a biomedical application. This nanocomposite was integrated with polydimethylsiloxane (PDMS) microchannels, which were constructed using the photolithographic technique. A structural and morphological characterization of the fabricated microfluidics chip, which was functionalized with a bienzyme containing cholesterol oxidase (ChOx) and cholesterol esterase (ChEt), was accomplished using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy. The XPS studies revealed that 9.3% of the carboxyl (COOH) groups present in the nNiO-MWCNT composite are used to form amide bonds with the NH 2 groups of the bienzyme. The response studies on this nanobiochip reveal good reproducibility and selectivity and a high sensitivity of 2.2 mA/mM/cm 2 . This integrated microfluidics biochip provides a promising low-cost platform for the rapid detection of biomolecules using minute samples.