Co-localization of corticotropin-releasing hormone with glutamate decarboxylase and calcium-binding proteins in infant rat neocortical interneurons

Corticotropin releasing hormone (CRH) has been localized to interneurons of the mammalian cerebral cortex, but these neurons have not been fully characterized. The present study determined the extent of co-localization of CRH with glutamate decarboxylase (GAD) and calcium-binding proteins in the inf...

Full description

Saved in:
Bibliographic Details
Published in:Experimental brain research 1998-12, Vol.123 (3), p.334-340
Main Authors: YAN, X.-X, BARAM, T. Z, GERTH, A, SCHULTZ, L, RIBAK, C. E
Format: Article
Language:English
Subjects:
ACTH
Animals
Animals, Newborn
Biological and medical sciences
Brain
Calbindin 2
Calbindins
Calcium-Binding Proteins - analysis
Corticotropin-Releasing Hormone - analysis
Development. Senescence. Regeneration. Transplantation
Fundamental and applied biological sciences. Psychology
GABA
Glutamate
Glutamate decarboxylase
Glutamate Decarboxylase - analysis
Hormones
Immunohistochemistry
Interneurons - chemistry
Neocortex - chemistry
Neocortex - cytology
Nerve Tissue Proteins - analysis
Neurons
Parvalbumins - analysis
Proteins
Rats
Rats, Sprague-Dawley
S100 Calcium Binding Protein G - analysis
Vertebrates: nervous system and sense organs
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Corticotropin releasing hormone (CRH) has been localized to interneurons of the mammalian cerebral cortex, but these neurons have not been fully characterized. The present study determined the extent of co-localization of CRH with glutamate decarboxylase (GAD) and calcium-binding proteins in the infant rat neocortex using immunocytochemistry. CRH-immunoreactive (ir) neurons were classified into two major groups. The first group was larger and consisted of densely CRH-immunostained small bipolar cells, predominantly localized to layers II and III. The second group of CRH-ir cells was lightly labeled and included multipolar neurons mainly found in deep cortical layers. Co-localization studies indicated that the vast majority of CRH-ir neurons, including both bipolar and multipolar types, was co-immunolabeled for GAD-65 and GAD-67. Most multipolar, but only some bipolar, CRH-ir neurons also contained parvalbumin, while CRH-ir neurons rarely contained calbindin or calretinin. These results indicate that virtually all CRH-ir neurons in the rat cerebral cortex are GABAergic. Furthermore, since parvalbumin is expressed by cortical basket and chandelier cells, the co-localization of CRH and parvalbumin suggests that some cortical CRH-ir neurons may belong to these two cell types.
ISSN:0014-4819
1432-1106
DOI:10.1007/s002210050576