Development of a rat schematic eye from in vivo biometry and the correction of lateral magnification in SD-OCT imaging

• Published in: Investigative ophthalmology & visual science 2013-09, Vol.54 (9), p.6446-6455
• Main Authors: Lozano, Diana C, Twa, Michael D
• Format: Article
• Language: English
• Subjects: Aging; Animals; Biometry - methods; Male; Nerve Fibers; Optic Disk - cytology; Rats; Rats, Inbred BN; Retinal Ganglion Cells - cytology; Tomography, Optical Coherence - methods
• ISSN: 1552-5783; 0146-0404; 1552-5783
• DOI: 10.1167/iovs.13-12575

Optical magnification in optical coherence tomography (OCT) depends on ocular biometric parameters (e.g., axial length). Biometric differences between eyes will influence scan location. A schematic model eye was developed to compensate for lateral magnification in OCT images of the healthy rat. Spectral-domain optical coherence tomography images were acquired in 19 eyes of 19 brown Norway rats. Images were scaled using the OCT instrument's built-in scaling function and by calculating the micron per degree from schematic model eyes developed from in vivo biometry (immersion A-scan and videokeratometry). Mean total retinal thickness was measured 500 μm away from the optic nerve head and optic nerve head diameter was measured. Corneal curvature, lens thickness, and axial length were modified to calculate their effects on OCT scan location and total retinal thickness. Mean total retinal thickness increased by 21 μm and the SD doubles when images were scaled with the Built-in scaling (222 ± 13 μm) compared with scaling with individual biometric parameters (201 ± 6 μm). Optic nerve head diameter was three times larger when images were scaled with the Built-in scaling (925 ± 97 μm) than the individual biometric parameters (300 ± 27 μm). Assuming no other change in biometric parameters, total retinal thickness would decrease by 37 μm for every millimeter increase in anterior chamber depth due to changes in ocular lateral magnification and associated change in scan location. Scaling SD-OCT images with schematic model eyes derived from individual biometric data is important. This approach produces estimates of retinal thickness and optic nerve head size that are in good agreement with previously reported measurements.