Effects of speckle/pixel size ratio on temporal and spatial speckle-contrast analysis of dynamic scattering systems: Implications for measurements of blood-flow dynamics

Laser Speckle Contrast Imaging (LSCI) is an optical technique used to generate blood flow maps with high spatial and temporal resolution. It is well known that in LSCI, the speckle size must exceed the Nyquist criterion to maximize the speckle's pattern contrast. In this work, we study experime...

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Bibliographic Details
Published in:Biomedical optics express 2013, Vol.4 (10), p.1883-1889
Main Authors: Ramirez-San-Juan, J C, Mendez-Aguilar, E, Salazar-Hermenegildo, N, Fuentes-Garcia, A, Ramos-Garcia, R, Choi, B
Format: Article
Language:English
Subjects:
Speckle Imaging and Diagnostics
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Summary:Laser Speckle Contrast Imaging (LSCI) is an optical technique used to generate blood flow maps with high spatial and temporal resolution. It is well known that in LSCI, the speckle size must exceed the Nyquist criterion to maximize the speckle's pattern contrast. In this work, we study experimentally the effect of speckle-pixel size ratio not only in dynamic speckle contrast, but also on the calculation of the relative flow speed for temporal and spatial analysis. Our data suggest that the temporal LSCI algorithm is more accurate at assessing the relative changes in flow speed than the spatial algorithm.
ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.4.001883