Kinetics of drug release from a biodegradable local drug delivery system and its effect on Porphyromonas gingivalis isolates: An in vitro study

Background: Conventional anti-microbial therapy largely consisted of systemic administration of various drugs effective against periodontal pathogens, but fraught with several problems. Based on the concept of local drug delivery a bioresorbable device made of pure fibrillar collagen has been develo...

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Published in:Journal of Indian Society of Periodontology 2013-07, Vol.17 (4), p.429-434
Main Authors: Vijayalashmi, Ranganathan, Ravindranath, Sabitha, Jayakumar, Nadathur, Vargheese, Sheeja, Kumaraswamy, Kikkeri
Format: Article
Language:English
Subjects:
Bacteria
Collagen
Dosage and administration
Drug delivery systems
Drug therapy
Drugs
Gram-negative bacteria
Health aspects
Methods
Original
Periodontal disease
Statistical analysis
Tetracycline
Tetracyclines
Vehicles
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Summary:Background: Conventional anti-microbial therapy largely consisted of systemic administration of various drugs effective against periodontal pathogens, but fraught with several problems. Based on the concept of local drug delivery a bioresorbable device made of pure fibrillar collagen has been developed. The aim of this study was to study the release of Tetracycline from this collagen fiber (Type I collagen) impregnated with Tetracycline and its antibacterial activity against Porphyromonas gingivalis. Materials and Methods: Porphyromonas gingivalis was isolated from plaque samples of chronic periodontitis patients by using a CO 2 incubator. DNA isolation was done followed by polymerase chain reaction (PCR) amplification to confirm the presence of bacteria. The release pattern of Tetracycline was assessed for a period of 10 days in water (group I) and Serum inoculated with Porphyromonas gingivalis (group II). Results: A significant presence of Tetracycline on all days in Group I and group II and the zone of inhibition was also present in both groups with a steady decline from day 1 to day 10. Conclusion: Since the results were well within the therapeutic concentration of drug required to inhibit the growth of gram -ve bacteria (Porphyromonas gingivalis), this bioresorbable Tetracycline fiber has the potential for clinical application.
ISSN:0972-124X
0975-1580
DOI:10.4103/0972-124X.118311