Reinfection studies of canine echinococcosis and role of dogs in transmission of Echinococcus multilocularis in Tibetan communities, Sichuan, China

• Published in: Parasitology 2013-11, Vol.140 (13), p.1685-1692
• Main Authors: MOSS, J. E., CHEN, X., LI, T., QIU, J., WANG, Q., GIRAUDOUX, P., ITO, A., TORGERSON, P. R., CRAIG, P. S.
• Format: Article
• Language: English
• Subjects: Animals; Anthelmintics - therapeutic use; Antigens, Helminth - genetics; Disease Vectors; Dog Diseases - drug therapy; Dog Diseases - epidemiology; Dog Diseases - parasitology; Dog Diseases - transmission; Dogs; Domestic animals; Echinococcosis - drug therapy; Echinococcosis - epidemiology; Echinococcosis - transmission; Echinococcosis - veterinary; Echinococcus granulosus - genetics; Echinococcus granulosus - isolation & purification; Echinococcus multilocularis - genetics; Echinococcus multilocularis - isolation & purification; Enzyme-Linked Immunosorbent Assay; Epidemiological Monitoring - veterinary; Feces - parasitology; Female; Humans; Life Sciences; Male; Microbiology and Parasitology; Parasite Egg Count; Parasitology; Polymerase Chain Reaction; Praziquantel - therapeutic use; Prevalence; Recurrence; Species Specificity; Tibet - epidemiology
• ISSN: 0031-1820; 1469-8161
• DOI: 10.1017/S0031182013001200

In the eastern Tibetan plateau both human cystic and alveolar echinococcosis (AE) caused by infection with Echincoccus granulosus or Echinococcus multilocularis, respectively are highly endemic. The domestic dog plays a key role in zoonotic transmission in this region. Our primary objective was to investigate the role of domestic dogs in maintaining transmission of E. multilocularis in Shiqu county, Sichuan. A cohort of 281 dogs was followed up over one year after a single treatment with praziquantel followed by re-infection surveillance at 2, 5 and 12 months post-treatment. Faecal samples were tested by an Echinococcus genus-specific coproantigen ELISA and two species-specific copro-PCR tests. Total Echinococcus coproantigen prevalence in Shiqu at baseline was 21% and 9·6% after 2 months. E. multilocularis copro-PCR was positive in 11·2% of dogs before treatment (vs 3·6% with E. granulosus copro-DNA), 2·9% at 2 months post-treatment, and 0% at 5 month and 12 months. The results suggest that dogs may have the potential to maintain E. multilocularis transmission within local pastoral communities, and thus dog dosing could be an effective strategy to reduce transmission of E. multilocularis as well as E. granulosus in these co-endemic Tibetan communities.