Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy

Surgery is an essential component in the treatment of brain tumors. However, delineating tumor from normal brain remains a major challenge. We describe the use of stimulated Raman scattering (SRS) microscopy for differentiating healthy human and mouse brain tissue from tumor-infiltrated brain based...

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Bibliographic Details
Published in:Science translational medicine 2013-09, Vol.5 (201), p.201ra119-201ra119
Main Authors: Ji, Minbiao, Orringer, Daniel A, Freudiger, Christian W, Ramkissoon, Shakti, Liu, Xiaohui, Lau, Darryl, Golby, Alexandra J, Norton, Isaiah, Hayashi, Marika, Agar, Nathalie Y R, Young, Geoffrey S, Spino, Cathie, Santagata, Sandro, Camelo-Piragua, Sandra, Ligon, Keith L, Sagher, Oren, Xie, X Sunney
Format: Article
Language:English
Subjects:
Animals
Brain - pathology
Brain Neoplasms - diagnosis
Brain Neoplasms - pathology
Color
Eosine Yellowish-(YS) - chemistry
Glioblastoma - diagnosis
Glioblastoma - pathology
Glioma
Hematoxylin - chemistry
Humans
Mice
Microscopy - methods
Neoplasm Transplantation
Observer Variation
Reproducibility of Results
Spectrum Analysis, Raman - methods
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Summary:Surgery is an essential component in the treatment of brain tumors. However, delineating tumor from normal brain remains a major challenge. We describe the use of stimulated Raman scattering (SRS) microscopy for differentiating healthy human and mouse brain tissue from tumor-infiltrated brain based on histoarchitectural and biochemical differences. Unlike traditional histopathology, SRS is a label-free technique that can be rapidly performed in situ. SRS microscopy was able to differentiate tumor from nonneoplastic tissue in an infiltrative human glioblastoma xenograft mouse model based on their different Raman spectra. We further demonstrated a correlation between SRS and hematoxylin and eosin microscopy for detection of glioma infiltration (κ = 0.98). Finally, we applied SRS microscopy in vivo in mice during surgery to reveal tumor margins that were undetectable under standard operative conditions. By providing rapid intraoperative assessment of brain tissue, SRS microscopy may ultimately improve the safety and accuracy of surgeries where tumor boundaries are visually indistinct.
ISSN:1946-6234
1946-6242
DOI:10.1126/scitranslmed.3005954