Urine concentrating mechanism in the inner medulla of the mammalian kidney: role of three-dimensional architecture

• Published in: Acta Physiologica 2011-07, Vol.202 (3), p.361-378
• Main Authors: Dantzler, W. H., Pannabecker, T. L., Layton, A. T., Layton, H. E.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Chlorides - metabolism; Computer Simulation; Fundamental and applied biological sciences. Psychology; Imaging, Three-Dimensional; Kidney Concentrating Ability - physiology; Kidney Medulla - anatomy & histology; Kidney Medulla - metabolism; Loop of Henle - anatomy & histology; Loop of Henle - metabolism; Mammals - anatomy & histology; Mammals - metabolism; mathematical models; Models, Biological; Models, Theoretical; renal inner medulla; Sodium - metabolism; three-dimensional reconstructions; Urine - chemistry; urine concentrating mechanism; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 1748-1708; 1748-1716
• DOI: 10.1111/j.1748-1716.2010.02214.x

The urine concentrating mechanism in the mammalian renal inner medulla (IM) is not understood, although it is generally considered to involve countercurrent flows in tubules and blood vessels. A possible role for the three‐dimensional relationships of these tubules and vessels in the concentrating process is suggested by recent reconstructions from serial sections labelled with antibodies to tubular and vascular proteins and mathematical models based on these studies. The reconstructions revealed that the lower 60% of each descending thin limb (DTL) of Henle’s loops lacks water channels (aquaporin‐1) and osmotic water permeability and ascending thin limbs (ATLs) begin with a prebend segment of constant length. In the outer zone of the IM (i) clusters of coalescing collecting ducts (CDs) form organizing motif for loops of Henle and vasa recta; (ii) DTLs and descending vasa recta (DVR) are arrayed outside CD clusters, whereas ATLs and ascending vasa recta (AVR) are uniformly distributed inside and outside clusters; (iii) within CD clusters, interstitial nodal spaces are formed by a CD on one side, AVR on two sides, and an ATL on the fourth side. These spaces may function as mixing chambers for urea from CDs and NaCl from ATLs. In the inner zone of the IM, cluster organization disappears and half of Henle’s loops have broad lateral bends wrapped around terminal CDs. Mathematical models based on these findings and involving solute mixing in the interstitial spaces can produce urine slightly more concentrated than that of a moderately antidiuretic rat but no higher.