Natural killer cell‐mediated contact sensitivity develops rapidly and depends on interferon‐α, interferon‐γ and interleukin‐12

Summary Natural killer (NK) cell‐mediated contact sensitivity was recently described in mice. Here, we confirm NK cell‐mediated contact sensitivity (CS) in SCID and RAG1−/− mice but not in SCIDbeige mice, which have non‐functional NK cells that lack NK cell granules. NK cell‐mediated CS was transfer...

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Bibliographic Details
Published in:Immunology 2013-09, Vol.140 (1), p.98-110
Main Authors: Majewska‐Szczepanik, Monika, Paust, Silke, Andrian, Ulrich H., Askenase, Philip W., Szczepanik, Marian
Format: Article
Language:English
Subjects:
Adaptive Immunity
Adoptive Transfer
Animals
contact sensitivity
cytokines
Dermatitis, Contact - etiology
Dermatitis, Contact - immunology
Female
Immunity, Innate
Interferon-alpha - metabolism
Interferon-gamma - metabolism
Interleukin-12 - metabolism
Killer Cells, Natural - immunology
Killer Cells, Natural - pathology
Liver - immunology
Liver - pathology
liver mononuclear cells
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred CBA
Mice, Knockout
Mice, SCID
natural killer cells
Original
Receptors, CXCR - metabolism
Receptors, CXCR6
Time Factors
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Summary:Summary Natural killer (NK) cell‐mediated contact sensitivity was recently described in mice. Here, we confirm NK cell‐mediated contact sensitivity (CS) in SCID and RAG1−/− mice but not in SCIDbeige mice, which have non‐functional NK cells that lack NK cell granules. NK cell‐mediated CS was transferred by liver mononuclear cells and the DX5+ fraction of liver cells, confirming that NK cells mediate CS in the absence of T and B cells. Participation of NKT cells and B‐1 cells was ruled out using Jα18−/− and JH−/− mice, respectively. Remarkably, NK cell‐mediated CS was observed just 1 hr after immunization and was detectable as early as 30 min after challenge. Further, we examined cytokine requirements for NK cell‐mediated CS, and found that liver mononuclear cells from interleukin‐12−/−, interferon‐γ−/− and interferon‐α receptor−/− donors fail to transfer NK cell‐mediated CS to naive hosts. Our studies clearly show that dinitrofluorobenzene sensitized NK cells mediate very rapid, antigen‐specific cell‐mediated immunity, with features of both innate and acquired immune responses.
ISSN:0019-2805
1365-2567
DOI:10.1111/imm.12120