Gene Expression and Pulmonary Toxicity of Chitosan-graft- Polyethylenimine as Aerosol Gene Carrier

Chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer has been used for the improvement of low transfection efficiency of chitosan. The present study aims to test the pulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposed to aerosol containing green-fluorescent...

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Published in:Iranian journal of pharmaceutical research : IJPR 2013, Vol.12 (2), p.281-286
Main Authors: Kwon, Jung-Taek, Jiang, Hu-Lin, Minai-Tehrani, Arash, Gyu Woo, Chang, Choi, Mansoo, Cho, Chong-Su, Kim, Yeon-Soo, Cho, Myung-Haing
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container_end_page 286
container_issue 2
container_start_page 281
container_title Iranian journal of pharmaceutical research : IJPR
container_volume 12
creator Kwon, Jung-Taek
Jiang, Hu-Lin
Minai-Tehrani, Arash
Gyu Woo, Chang
Choi, Mansoo
Cho, Chong-Su
Kim, Yeon-Soo
Cho, Myung-Haing
description Chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer has been used for the improvement of low transfection efficiency of chitosan. The present study aims to test the pulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposed to aerosol containing green-fluorescent protein (GFP)-polyethylenimine (PEI) or GFP-CHI-g-PEI complexes for 30 min during the development of our nose-only exposure chamber (NOEC) system. CHI-g-PEI-mediated aerosol delivery demonstrated 15.65% enhancement of the fluorescence intensity. Compared to PEI, CHI-g-PEI showed no significant pulmonary toxicity. In summary, using CHI-g-PEI is safe and shows high transfection in aerosol gene delivery to animals, and enhanced efficiency was achieved through our aerosol gene delivery system. Therefore, CHI-g-PEI and this system would be applicable to future study for aerosol gene therapy.
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title Gene Expression and Pulmonary Toxicity of Chitosan-graft- Polyethylenimine as Aerosol Gene Carrier
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