Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system

We adapted the CRISPR-Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the ger...

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Bibliographic Details
Published in:Genetics (Austin) 2013-11, Vol.195 (3), p.1181-1185
Main Authors: Tzur, Yonatan B, Friedland, Ari E, Nadarajan, Saravanapriah, Church, George M, Calarco, John A, Colaiácovo, Monica P
Format: Article
Language:English
Subjects:
Animals
Caenorhabditis elegans
Caenorhabditis elegans - genetics
CRISPR-Cas Systems
DNA Breaks, Double-Stranded
DNA, Helminth - genetics
Gene Targeting
Genetic Engineering
Genetic recombination
Genetic research
Genome, Helminth
Genomes
Genomics
Homologous Recombination
Mutagenesis
Mutagenesis, Insertional
Parasites
RNA Editing - genetics
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Summary:We adapted the CRISPR-Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans.
ISSN:1943-2631
0016-6731
1943-2631
DOI:10.1534/genetics.113.156075