Exciting prospects for precise engineering of Caenorhabditis elegans genomes with CRISPR/Cas9

With remarkable speed, the CRISPR-Cas9 nuclease has become the genome-editing tool of choice for essentially all genetically tractable organisms. Targeting specific DNA sequences is conceptually simple because the Cas9 nuclease can be guided by a single, short RNA (sgRNA) to introduce double-strand...

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Bibliographic Details
Published in:Genetics (Austin) 2013-11, Vol.195 (3), p.635-642
Main Author: Frøkjær-Jensen, Christian
Format: Article
Language:English
Subjects:
Animals
Caenorhabditis elegans
Caenorhabditis elegans - genetics
CRISPR-Cas Systems
Genetic engineering
Genetic research
Genome, Helminth
Genomes
Humans
Protocol
RNA Editing - genetics
Worms
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Summary:With remarkable speed, the CRISPR-Cas9 nuclease has become the genome-editing tool of choice for essentially all genetically tractable organisms. Targeting specific DNA sequences is conceptually simple because the Cas9 nuclease can be guided by a single, short RNA (sgRNA) to introduce double-strand DNA breaks (DSBs) at precise locations. Here I contrast and highlight protocols recently developed by eight different research groups, six of which are published in GENETICS, to modify the Caenorhabditis elegans genome using CRISPR/Cas9. This reverse engineering tool levels the playing field for experimental geneticists.
ISSN:1943-2631
0016-6731
1943-2631
DOI:10.1534/genetics.113.156521