Semi-quantitative scoring of an immunochromatographic test for circulating filarial antigen

The value of a semi-quantitative scoring of the filarial antigen test (Binax Now Filariasis card test, ICT) results was evaluated during a field survey in the Republic of Congo. One hundred and thirty-four (134) of 774 tests (17.3%) were clearly positive and were scored 1, 2, or 3; and 11 (1.4%) had...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2013-11, Vol.89 (5), p.916-918
Main Authors: Chesnais, Cédric B, Missamou, François, Pion, Sébastien D S, Bopda, Jean, Louya, Frédéric, Majewski, Andrew C, Weil, Gary J, Boussinesq, Michel
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Animals
Antigens, Helminth - blood
Cell Count
Child
Child, Preschool
Congo - epidemiology
Enzyme-Linked Immunosorbent Assay
Filariasis - blood
Filariasis - diagnosis
Filariasis - epidemiology
Filariasis - parasitology
Humans
Microfilariae - immunology
Microfilariae - isolation & purification
Middle Aged
Reagent Kits, Diagnostic
Research Design
Short Report
Wuchereria bancrofti - immunology
Wuchereria bancrofti - isolation & purification
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Summary:The value of a semi-quantitative scoring of the filarial antigen test (Binax Now Filariasis card test, ICT) results was evaluated during a field survey in the Republic of Congo. One hundred and thirty-four (134) of 774 tests (17.3%) were clearly positive and were scored 1, 2, or 3; and 11 (1.4%) had questionable results. Wuchereria bancrofti microfilariae (mf) were detected in 41 of those 133 individuals with an ICT test score ≥ 1 who also had a night blood smear; none of the 11 individuals with questionable ICT results harbored night mf. Cuzick's test showed a significant trend for higher microfilarial densities in groups with higher ICT scores (P < 0.001). The ICT scores were also significantly correlated with blood mf counts. Because filarial antigen levels provide an indication of adult worm infection intensity, our results suggest that semi-quantitative reading of the ICT may be useful for grading the intensity of filarial infections in individuals and populations.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.13-0245